Plant Ribosomal Proteins, RPL12 and RPL19, Play a Role in Nonhost Disease Resistance against Bacterial Pathogens

Frontiers in Plant Science
Satish NagarajKirankumar S Mysore

Abstract

Characterizing the molecular mechanism involved in nonhost disease resistance is important to understand the adaptations of plant-pathogen interactions. In this study, virus-induced gene silencing (VIGS)-based forward genetics screen was utilized to identify genes involved in nonhost resistance in Nicotiana benthamiana. Genes encoding ribosomal proteins, RPL12 and RPL19, were identified in the screening. These genes when silenced in N. benthamiana caused a delay in nonhost bacteria induced hypersensitive response (HR) with concurrent increase in nonhost bacterial multiplication. Arabidopsis mutants of AtRPL12 and AtRPL19 also compromised nonhost resistance. The studies on NbRPL12 and NbRPL19 double silenced plants suggested that both RPL12 and RPL19 act in the same pathway to confer nonhost resistance. Our work suggests a role for RPL12 and RPL19 in nonhost disease resistance in N. benthamiana and Arabidopsis. In addition, we show that these genes also play a minor role in basal resistance against virulent pathogens.

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Citations

May 21, 2016·Frontiers in Plant Science·Alban LaunayMathilde Fagard
Jul 17, 2020·Frontiers in Plant Science·Federico Martinez-SeidelJoachim Kopka
Dec 22, 2019·International Journal of Molecular Sciences·Mingqing DangYajuan Qian
Nov 9, 2018·Frontiers in Microbiology·Diane Bortolamiol-BécetVéronique Ziegler-Graff
Mar 22, 2019·Frontiers in Plant Science·Ramu S VemannaUdayakumar Makarla
Feb 24, 2019·International Journal of Molecular Sciences·Yuying WangYihong Ge
Nov 25, 2020·Molecular Plant-microbe Interactions : MPMI·Vemanna S RamuKirankumar S Mysore
Apr 15, 2021·Molecular Plant-microbe Interactions : MPMI
May 11, 2021·Frontiers in Plant Science·Wei XiongBeixin Mo
Jun 26, 2021·The Plant Journal : for Cell and Molecular Biology·Sekhar KambakamMadan K Bhattacharyya
Oct 17, 2021·Communications Biology·Abhisek ChakrabortyVineet K Sharma

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Datasets Mentioned

BETA
JZ764382

Methods Mentioned

BETA
transgenic

Software Mentioned

primer quest
BLAST

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