Plant tRNA nucleotidyltransferase : I. Isolation and purification of tRNA nucleotidyltransferase from Lupinus luteus seeds

H CudnyJ Kaczkowski


A method for purification of tRNA nucleotidyltransferase from Lupinus luteus L. seeds on a preparative scale is presented. This involved ammonium sulfate fractionation and chromatography on DEAE-cellulose, hydroxylapatite, and QAE-Sephadex A-50. The final enzyme prepartion was apparently homogeneous and a 2000-fold purification was achieved. Data presented suggest the existence of only one form of tRNA nucleotidyltransferase in dry lupin seeds.


Jan 1, 1973·Progress in Nucleic Acid Research and Molecular Biology·M P Deutscher
Sep 24, 1971·European Journal of Biochemistry·H SternbachF Cramer
Dec 14, 1970·Biochimica Et Biophysica Acta·D S CarreF Chapeville

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