Plant tRNA nucleotidyltransferase : I. Isolation and purification of tRNA nucleotidyltransferase from Lupinus luteus seeds

Planta
H CudnyJ Kaczkowski

Abstract

A method for purification of tRNA nucleotidyltransferase from Lupinus luteus L. seeds on a preparative scale is presented. This involved ammonium sulfate fractionation and chromatography on DEAE-cellulose, hydroxylapatite, and QAE-Sephadex A-50. The final enzyme prepartion was apparently homogeneous and a 2000-fold purification was achieved. Data presented suggest the existence of only one form of tRNA nucleotidyltransferase in dry lupin seeds.

References

Jan 1, 1973·Progress in Nucleic Acid Research and Molecular Biology·M P Deutscher
Sep 24, 1971·European Journal of Biochemistry·H SternbachF Cramer
Dec 14, 1970·Biochimica Et Biophysica Acta·D S CarreF Chapeville

Related Concepts

Ammonium Sulfate
Chromatography
DEAE-Cellulose
Sephadex
TRNA adenylyltransferase
Durapatite
Isolation Aspects
Lupinus
Lupinus luteus
Radiotherapy Fractionation

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