PMID: 12757916May 22, 2003Paper

Plant virus cDNA chip hybridization for detection and differentiation of four cucurbit-infecting Tobamoviruses

Journal of Virological Methods
Gung Pyo LeeKi Hyun Ryu

Abstract

A plant virus cDNA chip was developed by using viral cDNA clones and microarray technology. The cDNA chip was designed for detection and differentiation of the four species of selected cucurbit-infecting tobamoviruses [target viruses: Cucumber green mottle mosaic virus (CGMMV); Cucumber fruit mottle mosaic virus (CFMMV); Kyuri green mottle mosaic virus (KGMMV); and Zucchini green mottle mosaic virus (ZGMMV)]. The chip consisted of cDNA clones of the four cucurbit-infecting tobamoviruses, two target-related tobamoviruses, and another three unrelated plant viruses. Polymerase chain reaction products were amplified from the selected cDNA clones and arrayed onto slide glass. The cDNA chip, which was called cucurbit-virus chip, detected successfully specific target viruses. When applied to probes made from ZGMMV-infected samples, ZGMMV reacted strongly with its homologous cDNA and moderately reacted with KGMMV and CFMMV, while it did not react with CGMMV on the same chip. CGMMV probe gave strong signal intensity to its homologous cDNA spot and weakly reacted with ZGMMV, KGMMV, and CFMMV. The signal intensity of all combinations of probe and target was correlated significantly with nucleotide sequence identities between the probes an...Continue Reading

References

Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P GoeletJ Karn
Mar 12, 2002·Virus Research·Gary Vernet

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Citations

Aug 19, 2007·Annual Review of Phytopathology·Neil BoonhamRick Mumford
Nov 17, 2009·Journal of Virological Methods·Esteban A EngelPablo D T Valenzuela
Oct 9, 2007·Journal of Virological Methods·Graziella PasquiniHenryk Czosnek
Sep 6, 2005·The Veterinary Journal·Mohit K BaxiDirk Deregt
Feb 9, 2013·Journal of Virological Methods·Kristina FlorschützGotthard Kunze
Jun 20, 2012·Journal of Virological Methods·Antonio Tiberini, Marina Barba
Feb 8, 2011·Journal of Virological Methods·Mogens Nicolaisen
Sep 27, 2018·Frontiers in Microbiology·Vicente PallásDelano James
Jan 1, 2006·European Journal of Plant Pathology·Rick MumfordIan Barker

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