Plaque assay and cloning of scrub typhus rickettsiae in irradiated L-929 cells.

Journal of Clinical Microbiology
S C OaksF M Hetrick

Abstract

It was demonstrated that gamma-irradiated L-929 cells support plaque formation by three strains of Rickettsia tsutsugamushi and representative species of the spotted fever and typhus group rickettsiae. Sensitivity of the plaque assay for detection of viable scrub typhus rickettsiae was similar to that achieved with intraperitoneal inoculation of random-bred mice. The concentration of irradiated cells and the temperature and length of incubation were all found to affect plaque size. A technique combining terminal dilution and plaque purification was used to obtain clones of three strains of scrub typhus rickettsiae.

References

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Citations

Nov 1, 1979·Infection and Immunity·C A Nacy, J V Osterman
Jun 1, 1986·Infection and Immunity·D J Kelly, J C Rees
Apr 1, 1980·Infection and Immunity·G H EisenbergE H Stephenson
Jan 1, 1984·Infection and Immunity·B A PalmerT J Jerrells
Jan 1, 1984·Infection and Immunity·C S EisemannJ V Osterman
Aug 1, 1996·Journal of Clinical Microbiology·P F PolicastroT Hackstadt

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