Plaque assay and improved yield of human coronaviruses in a human rhabdomyosarcoma cell line.

Journal of Clinical Microbiology
O W SchmidtG E Kenny

Abstract

Propagation and plaque assay of human coronavirus prototypes were studied in two human cell lines: a diploid fetal tonsil (FT) and a heteroploid rhabdomyosarcoma (RD) cell lines. Plaques, observed within 2 to 3 days on FT cell monolayers with both 229E and OC43 viruses, appeared as colorless areas after staining with neutral red or crystal violet, whereas neutral red staining was required for visualization of plaques on RD cells. The plating efficiencies were approximately equal between the two cell lines, but virus assay by plaque formation was 15- to 30-fold more efficient than tube dilution assay with 50% endpoints. The discrepancy between 50% endpoint and plaque-forming unit values was striking and appeared to result from the fact that killing of cells (particularly RD cells) by coronaviruses was not accompanied by visible changes in the cells but killing was detected by the failure of infected cells to stain with a vital dye. The latent phase in one-step growth curves was 5 to 6 h for both viruses in either cell line, but the maximum yield of intracellular virus was reached in 18 to 20 h for FT cells and 24 to 28 h for RD cells. Virus release also differed between the two cell lines: in FT cells, the maximum yield of extra...Continue Reading

References

Sep 1, 1969·Cancer·R M McAllisterM B Gardner
Jan 1, 1966·Proceedings of the Society for Experimental Biology and Medicine·D Hamre, J J Procknow
Dec 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·K McIntoshR M Chanock
Feb 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·M K Cooney, G E Kenny
Jun 1, 1970·American Journal of Epidemiology·K McIntoshR M Chanock
Jan 1, 1970·Archiv für die gesamte Virusforschung·A F Bradburne
Aug 1, 1967·Journal of Virology·D HamreJ Mann
Jan 1, 1969·Archiv für die gesamte Virusforschung·A F Bradburne, D A Tyrrell
Nov 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·M BruckováR M Chanock
Jan 1, 1972·Archiv für die gesamte Virusforschung·A F Bradburne
Mar 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·R A BucknallR M Chanock
Apr 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·K McIntoshR M Chanock
Jun 5, 1965·British Medical Journal·D A TYRRELL, M L BYNOE

❮ Previous
Next ❯

Citations

Jun 1, 1981·Infection and Immunity·O W Schmidt, G E Kenny
Feb 1, 1982·Infection and Immunity·O W Schmidt, G E Kenny
Sep 1, 1980·Journal of Clinical Microbiology·M R MacnaughtonS Patterson
Jul 14, 2004·Biochemical and Biophysical Research Communications·Runtao HeXuguang Li
Jan 1, 1988·Journal of Tissue Culture Methods : Tissue Culture Association Manual of Cell, Tissue, and Organ Culture Procedures·Roger D Woods, Ronald D Wesley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.