Plaque-dependent morphological and electrophysiological heterogeneity of microglia in an Alzheimer's disease mouse model

Glia
Monika PlescherAnnett Halle

Abstract

Microglia, the central nervous system resident innate immune cells, cluster around Aβ plaques in Alzheimer's disease (AD). The activation phenotype of these plaque-associated microglial cells, and their differences to microglia distant to Aβ plaques, are incompletely understood. We used novel three-dimensional cell analysis software to comprehensively analyze the morphological properties of microglia in the TgCRND8 mouse model of AD in spatial relation to Aβ plaques. We found strong morphological changes exclusively in plaque-associated microglia, whereas plaque-distant microglia showed only minor changes. In addition, patch-clamp recordings of microglia in acute cerebral slices of TgCRND8 mice revealed increased K+ currents in plaque-associated but not plaque-distant microglia. Within the subgroup of plaque-associated microglia, two different current profiles were detected. One subset of cells displayed only increased inward currents, while a second subset showed both increased inward and outward currents, implicating that the plaque microenvironment differentially impacts microglial ion channel expression. Using pharmacological channel blockers, multiplex single-cell PCR analysis and RNA fluorescence in situ hybridization, we...Continue Reading

References

Jan 1, 1990·Acta Neuropathologica·J Wegiel, H M Wisniewski
Oct 1, 1989·Journal of Neuroimmunology·S ItagakiD Selkoe
Jan 1, 1993·Glia·H KettenmannW Walz
Jul 11, 2000·The European Journal of Neuroscience·C BoucseinC Nolte
Sep 26, 2000·American Journal of Physiology. Cell Physiology·T SchillingC Eder
Nov 18, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S A LyonsU Dirnagl
Mar 14, 2001·American Journal of Physiology. Cell Physiology·R KhannaL C Schlichter
Mar 22, 2002·Molecular and Cellular Neurosciences·Wolfgang SchröderChristian Steinhäuser
Jun 24, 2004·Neurobiology of Aging·Sherri DudalFrancine Gervais
Jun 2, 2005·Journal of Neuroscience Research·Claudia Eder
Aug 5, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Christopher B FordyceLyanne C Schlichter
Oct 24, 2006·Molecular and Cellular Neurosciences·Ulrike PannaschHelmut Kettenmann
Nov 10, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sonia FranciosiJames G McLarnon
Oct 30, 2007·Nature Neuroscience·Uwe-Karsten Hanisch, Helmut Kettenmann
Apr 18, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tristan BolmontMichael E Calhoun
Apr 25, 2008·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Richard A ArmstrongNigel J Cairns
Sep 12, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Elena AvignoneEtienne Audinat
Dec 26, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jessica Koenigsknecht-TalbooDavid M Holtzman
Jun 12, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gerald SeifertChristian Steinhäuser
Jan 21, 2010·Physiological Reviews·Hiroshi HibinoYoshihisa Kurachi
Mar 9, 2010·CNS & Neurological Disorders Drug Targets·Shweta Mandrekar-Colucci, Gary E Landreth
Mar 17, 2010·Microscopy Research and Technique·Ignacio Arganda-CarrerasCarlos Ortiz-De-Solorzano
Apr 8, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tomomi KiyotaTsuneya Ikezu
Jan 14, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jasmin K HefendehlMathias Jucker
Apr 30, 2011·Physiological Reviews·Helmut KettenmannAlexei Verkhratsky
Dec 15, 2011·The Journal of Molecular Diagnostics : JMD·Fay WangYuling Luo
May 10, 2013·Journal of Neuropathology and Experimental Neurology·Alberto Serrano-PozoBradley T Hyman
Sep 18, 2013·The Journal of General Physiology·David J CombsZhe Lu
Sep 5, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ye ZhangJian Qian Wu
Nov 2, 2014·Journal of Alzheimer's Disease : JAD·Srikant RangarajuAllan Levey
Dec 5, 2014·Glia·Tom Schilling, Claudia Eder

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Citations

Nov 18, 2018·British Journal of Pharmacology·Zachery MoorePeter J Crack
Jan 17, 2019·Annual Review of Immunology·Kevin BasslerJoachim L Schultze
Jun 28, 2019·Cells·Diana K Franco-BocanegraDelphine Boche
Apr 26, 2019·Annals of Clinical and Translational Neurology·Lee-Way JinIzumi Maezawa
Nov 30, 2018·Frontiers in Aging Neuroscience·Filippo UgoliniMaria Grazia Giovannini
Aug 6, 2020·Frontiers in Cellular Neuroscience·Jinqiang ZhangTao Zhou
Oct 17, 2018·Frontiers in Neuroscience·Laura TheiTalitha L Kerrigan
Jun 6, 2018·Journal of Alzheimer's Disease : JAD·Anne-Caroline SchmöleAndreas Zimmer
May 23, 2019·Acta Neuropathologica Communications·T L StephenC J Pike
Nov 11, 2019·Acta Neuropathologica Communications·Diana K Franco-BocanegraDelphine Boche
Nov 28, 2020·Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·I K MalashenkovaN A Didkovsky
Dec 17, 2020·International Journal of Molecular Sciences·Daniele LanaMaria Grazia Giovannini
Dec 16, 2020·Nature Reviews. Neurology·Fangda Leng, Paul Edison
Feb 24, 2020·Neurobiology of Aging·Christine KlausBettina Linnartz-Gerlach
Jun 1, 2021·Frontiers in Neurology·Marie J PietrowskiKevin Carvalho
Jun 10, 2021·Neuroscience Bulletin·Yang Zhang, Donghong Cui
Aug 7, 2021·Scientific Reports·Diana K Franco-BocanegraDelphine Boche
Aug 1, 2021·Clinical and Experimental Immunology·Wissam BeainoAlbert D Windhorst

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