Feb 1, 1954

Plaque formation and isolation of pure lines with poliomyelitis viruses

The Journal of Experimental Medicine
R DULBECCO, M VOGT

Abstract

Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single virus particle, defined as the virus unit that is unseparable by dilution. The plaques are due to the specific action of the virus since they are suppressed by type-specific antiserum. Pure virus lines were established by isolating the virus population produced in single plaques. These derived virus lines had the same morphological, serological, and pathogenic properties as the parent strain. High titer virus stocks, with titers up to 7 x 10(8) plaque-forming particles per ml., were obtained.

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  • Citations1873

Citations

Mentioned in this Paper

Benign Neoplasm of Testis
Pathogenic Organism
Neoplasm of Uncertain or Unknown Behavior of Testis
Virion
Immune Sera
Dental Plaque
Human poliovirus 3
Testis
Senile Plaques
Poliomyelitis

About this Paper

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