Plaque formation by avian infectious bronchitis virus in primary chick embryo fibroblast cells in the presence of trypsin

Archives of Virology
K Otsuki, M Tsubokura


Ten strains of avian infectious bronchitis virus (IBV) were titrated as plaque-forming units in primary chick embryo fibroblast cells. In the absence of trypsin, plaques were only formed by Beaudette-42 and Iowa-609 strains. When trypsin was incorporated in the overlay medium of cell monolayers, all the IBV strains tested produced plaques within 4 days after inoculation. Incorporation of 20-40 microgram of trypsin per ml of the overlay medium seemed to be suitable for plaque formation of IBV. A preliminary investigation was made of the mode of action of trypsin.


Nov 1, 1979·Journal of Clinical Microbiology·S Ramia, S A Sattar
Dec 1, 1975·Virology·H D KlenkJ Blödorn
Dec 1, 1974·The Journal of General Virology·G Appleyard, H B Maber
Sep 1, 1972·The Journal of General Virology·C H CunninghamK Nazerian
Nov 1, 1967·Proceedings of the Society for Experimental Biology and Medicine·G E Gifford, D G Klapper
Jan 1, 1968·Archiv für die gesamte Virusforschung·P E CameG Shimonaski
Mar 1, 1965·Journal of Bacteriology·R S SPENDLOVE, F L SCHAFFER

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