Plasma-activated medium-induced intracellular zinc liberation causes death of SH-SY5Y cells

Archives of Biochemistry and Biophysics
Hirokazu HaraTetsuo Adachi

Abstract

Plasma is an ionized gas consisting of ions, electrons, free radicals, neutral particles, and photons. Plasma-activated medium (PAM), which is prepared by the irradiation of cell-free medium with non-thermal atmospheric pressure plasma, induces cell death in various types of cancer cell. Since PAM contains reactive oxygen species (ROS), its anti-cancer effects are thought to be attributable to oxidative stress. Meanwhile, oxidative stress has been shown to induce the liberation of zinc (Zn(2+)) from intracellular Zn(2+) stores and to provoke Zn(2+)-dependent cell death. In this study, we thus examined whether Zn(2+) is involved in PAM-induced cell death using human neuroblastoma SH-SY5Y cells. Exposure to PAM triggered cell death in SH-SY5Y cells. The cell-permeable Zn(2+) chelator N,N,N',N'-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine (TPEN) protected against PAM-induced cell death. Zn(2+) imaging using the fluorescent Zn(2+) probe FluoZin-3 revealed that PAM elicited a rise of intracellular free Zn(2+). In addition, PAM stimulated PARP-1 activation, mitochondrial ROS generation, and the depletion of intracellular NAD(+) and ATP. These findings suggest that PAM-induced PARP-1 activation causes energy supply exhaustion. Moreov...Continue Reading

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Citations

Jul 30, 2016·Archives of Biochemistry and Biophysics·Tetsuo AdachiHirokazu Hara
Nov 3, 2017·Free Radical Research·Megumi ShimojiTetsuo Adachi
Feb 7, 2017·Journal of Clinical Biochemistry and Nutrition·Hiromasa Tanaka, Masaru Hori
Oct 25, 2020·Scientific Reports·Yi-Jing ChengYun-Chien Cheng
Oct 20, 2019·Archives of Biochemistry and Biophysics·Misaki NagayaTetsuo Adachi

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