Plasma and urine profiles of Delta9-tetrahydrocannabinol and its metabolites 11-hydroxy-Delta9-tetrahydrocannabinol and 11-nor-9-carboxy-Delta9-tetrahydrocannabinol after cannabis smoking by male volunteers to estimate recent consumption by athletes

Analytical and Bioanalytical Chemistry
Rudolf BrenneisenMatthias Kamber

Abstract

Since 2004, cannabis has been prohibited by the World Anti-Doping Agency for all sports competitions. In the years since then, about half of all positive doping cases in Switzerland have been related to cannabis consumption. In doping urine analysis, the target analyte is 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol (THC-COOH), the cutoff being 15 ng/mL. However, the wide urinary detection window of the long-term metabolite of Delta(9)-tetrahydrocannabinol (THC) does not allow a conclusion to be drawn regarding the time of consumption or the impact on the physical performance. The purpose of the present study on light cannabis smokers was to evaluate target analytes with shorter urinary excretion times. Twelve male volunteers smoked a cannabis cigarette standardized to 70 mg THC per cigarette. Plasma and urine were collected up to 8 h and 11 days, respectively. Total THC, 11-hydroxy-Delta(9)-tetrahydrocannabinol (THC-OH), and THC-COOH were determined after hydrolysis followed by solid-phase extraction and gas chromatography/mass spectrometry. The limits of quantitation were 0.1-1.0 ng/mL. Eight puffs delivered a mean THC dose of 45 mg. Plasma levels of total THC, THC-OH, and THC-COOH were measured in the ranges 0.2-59.1, 0.1-...Continue Reading

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Citations

Aug 9, 2013·Analytical and Bioanalytical Chemistry·Mario ThevisWilhelm Schänzer
Jul 27, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kang Mi LeeJaeick Lee
Dec 18, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Ju-Yeon MoonMan Ho Choi
Jan 29, 2014·Journal of Pharmaceutical and Biomedical Analysis·Camilla MontesanoRoberta Curini
May 2, 2020·Journal of Assisted Reproduction and Genetics·Malinda S LeeJanis H Fox
Jan 22, 2011·Drug Testing and Analysis·Mario ThevisWilhelm Schänzer
Sep 6, 2019·Journal of the National Comprehensive Cancer Network : JNCCN·Young D ChangDiane Portman
Apr 18, 2020·Bulletin de l'Académie nationale de médecine·P Kintz
Jun 22, 2014·Environmental Science & Technology·Frederic BeenPierre Esseiva
Aug 15, 2017·Environmental Science & Technology·Allison L MackieGraham A Gagnon

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