Plasma C5 glucose-to-2H2O ratio does not provide an accurate assessment of gluconeogenesis during hyperinsulinemic-euglycemic clamps in either nondiabetic or diabetic humans.

Diabetes
Rita BasuRobert Rizza

Abstract

Measurement of plasma C2 glucose enrichment is cumbersome. Therefore, the plasma C5 glucose-to-(2)H(2)O rather than the plasma C5-to-C2 glucose ratio commonly has been used to measure gluconeogenesis and glycogenolysis during hyperinsulinemic-euglycemic clamps. The validity of this approach is unknown. Ten nondiabetic and 10 diabetic subjects ingested (2)H(2)O the evening before study. The following morning, insulin was infused at a rate of 0.6 mU . kg(-1) . min(-1) and glucose was clamped at approximately 5.3 mmol/l for 5 h. Plasma C5 glucose, C2 glucose, and (2)H(2)O enrichments were measured hourly from 2 h onward. Plasma C2 glucose and plasma (2)H(2)O enrichment were equal in both groups before the clamp, resulting in equivalent estimates of gluconeogenesis and glycogenolysis. In contrast, plasma C2 glucose and plasma C5 glucose enrichments fell throughout the clamp, whereas plasma (2)H(2)O enrichment remained unchanged. Since the C5 glucose concentration and, hence, the C5 glucose-to-(2)H(2)O ratio is influenced by both gluconeogenesis and glucose clearance, whereas the C5-to-C2 glucose ratio is only influenced by gluconeogenesis, the C5 glucose-to-(2)H(2)O ratio overestimated (P < 0.01) gluconeogenesis during the clamp. T...Continue Reading

References

Jul 1, 1978·Metabolism: Clinical and Experimental·A D CherringtonM S Harris
Jan 1, 1995·The Journal of Clinical Investigation·B R LandauS C Kalhan
Jul 15, 1996·The Journal of Clinical Investigation·B R LandauS C Kalhan
Oct 6, 1997·The Journal of Clinical Investigation·S KalhanA O'Brien
Dec 20, 2000·American Journal of Physiology. Endocrinology and Metabolism·G BodenT P Stein
Jun 18, 2002·American Journal of Physiology. Endocrinology and Metabolism·Guenther BodenMaria Mozzoli
Nov 5, 2002·The Journal of Clinical Endocrinology and Metabolism·Amalia GastaldelliEle Ferrannini
Oct 1, 1956·The American Journal of Physiology·R STEELEN ALTSZULER
Feb 26, 2004·American Journal of Physiology. Endocrinology and Metabolism·Rita BasuRobert A Rizza
Aug 5, 2004·The Journal of Clinical Endocrinology and Metabolism·Amalia GastaldelliRalph A DeFronzo
Dec 15, 2005·The Journal of Clinical Endocrinology and Metabolism·Amalia GastaldelliEle Ferrannini
Jan 13, 2006·American Journal of Physiology. Endocrinology and Metabolism·Gideon AllickHans P Sauerwein
Sep 28, 2006·Diabetes Care·Amalia GastaldelliRalph A DeFronzo

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Citations

Nov 26, 2015·Diabetes·Stephanie T ChungMorey W Haymond
Oct 2, 2012·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Cristina BarosaRita Basu
Jul 23, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Ivan ViegasJohn G Jones
Jun 20, 2018·American Journal of Physiology. Endocrinology and Metabolism·Claire GaudichonJean-Philippe Godin
Feb 23, 2019·The Journal of Clinical Endocrinology and Metabolism·Devjit TripathyRalph A DeFronzo

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