Plasma CCL5 promotes EMT-medicated epirubicin-resistance in locally advanced breast cancer

Cancer Biomarkers : Section a of Disease Markers
Ge MaShui Wang

Abstract

Neoadjuvant chemotherapy (NCT) is the standard treatment for locally advanced breast cancer (LABC). Pathological complete response (pCR) is commonly used as a valid predictor of NCT long-term outcomes. Blood-based tumor biomarkers have the potential to predict response to NCT at early stage non-invasively. We believed plasma CCL5 could be a potential marker to predict NCT of LABC. Its efficiency and possible mechanism was studied in this work. Human Cytokine Antibody Microarray was applied to screen different cytokine concentration in plasma between low histological regression (Low-R) and high histological regression (High-R) patients. LABC patients were divided into two groups according to pathological reactivity. The concentration of plasma CCL5 in different groups was determined by ELISA analysis. CCK8 assay was performed to analyze epirubicin susceptibility of breast cancer cells. Transwell assay was performed to determine the effect of CCL5 on breast cancer cells' migration and invasion. qRT-PCR and western blot were used to verify the EMT (epithelial-mesenchymal transition) markers in CCL5-treated and epirubicin-treated breast cancer cells. The concentration of plasma CCL5 of Low-R group was higher than High-R group befor...Continue Reading

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Citations

Sep 9, 2021·Bioscience Reports·Jiahui WeiJiabao Zhang

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Methods Mentioned

BETA
Antibody Array
biopsy
antibody
enzyme-linked immunosorbent assay
PCR
protein assay
electrophoresis
ELISA

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