Plasma circular RNA profiling of patients with gastric cancer and their droplet digital RT-PCR detection

Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte
Tianwen LiJunming Guo

Abstract

To observe the diagnostic values of circular RNAs (circRNAs), their expression profiles between gastric cancer patients' plasma and healthy controls were first assessed by circRNA microarray. Then, circRNA levels were measured by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and RT-droplet digital PCR (RT-ddPCR), respectively. A total of 343 differentially expressed circRNAs were found. The top 10 elevated circRNAs in patients were hsa_circ_0088300, hsa_circ_0075825, hsa_circ_0019172, hsa_circ_0000220, hsa_circ_0035277, hsa_circ_0000301, hsa_circ_0000189, hsa_circ_0090080, hsa_circ_0001888, and hsa_circ_0001874. The top 10 reduced circRNAs were hsa_circ_0004771, hsa_circ_0001190, hsa_circ_0061276, hsa_circ_0092337, hsa_circ_0058495, hsa_circ_0061274, hsa_circ_0075829, hsa_circ_0080845, hsa_circ_0001006, and hsa_circ_0003764. In cancer and dysplasia tissues, hsa_circ_0001017 and hsa_circ_0061276 were downregulated. Their levels were significantly associated with distal metastasis. The area under receiver operating characteristic curve in combinative use was 0.966 with 95.5% sensitivity and 95.7% specificity. Patients with low plasma hsa_circ_0001017 or hsa_circ_0061276 had a much shorter overall survival...Continue Reading

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Citations

Nov 29, 2017·Genes·Shahnaz Haque, Lorna W Harries
May 23, 2018·Journal of Clinical Laboratory Analysis·Ting YaoBingxiu Xiao
Jun 2, 2018·Cancer Medicine·Liyun FuJunming Guo
Aug 2, 2018·Journal of Hematology & Oncology·Yiting GengChangping Wu
Aug 22, 2018·Journal of Cellular Biochemistry·Hongbo LiYonggang Fan
May 29, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Antonia FranzAnnika Fendler
Jun 7, 2019·Journal of Clinical Laboratory Analysis·Jiaxin GeXinjun Zhang
Nov 10, 2018·Current Gene Therapy·Shuai FangZhaohui Gong
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Jan 11, 2020·Expert Review of Gastroenterology & Hepatology·Yao RuanJunming Guo
Aug 29, 2019·International Journal of Cancer. Journal International Du Cancer·Jian YuWei-Ping Zhou
Feb 20, 2020·Journal of Cellular and Molecular Medicine·Zhi-Hong ZongYang Zhao
Nov 14, 2019·Journal of Clinical Laboratory Analysis·Xiuchong YuJunming Guo
Oct 30, 2019·Cellular and Molecular Life Sciences : CMLS·Rong LiWenrong Xu
May 6, 2020·Nature Reviews. Molecular Cell Biology·Ling-Ling Chen
May 19, 2020·Journal of Clinical Laboratory Analysis·Yezhao WangJunming Guo
Aug 23, 2019·Pathology Oncology Research : POR·Yongfu ShaoJunming Guo
Jun 26, 2020·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·F KhanipouyaniM R Fattahi
Jul 11, 2020·Chinese Medical Journal·Xue-Wei LiJun Xu
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Sep 15, 2020·Frontiers in Oncology·Ting Wang, Kun-He Zhang
Jun 9, 2020·Frontiers in Molecular Biosciences·Peijing ZhangMing Chen

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Datasets Mentioned

BETA
GES-1
GSE89143
GSE93541

Methods Mentioned

BETA
PCR
electrophoresis
circRNA Array

Software Mentioned

QuantaSoft
GenePix Pro
Statistical Program for Social Sciences ( SPSS )
R
Arraystar
BLAST
R Core Team
R packages
Primer

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