PMID: 6407789Mar 1, 1983Paper

Plasma contamination accounts for thyroid hormone binding in nuclear protein extracts from human mononuclear cells

Clinical Endocrinology
G F MaberlyK V Waite

Abstract

Plasma protein contamination accounted for high affinity thyroid hormone binding to a nuclear protein extract from human blood mononuclear cells. The degree of contamination was directly related to the number of times the cells were washed before the nuclear protein was extracted. After three washes, no specific binding was detected. Plasma contamination was estimated to be 0.3-0.5 microliter/assay tube. The TBG concentration in this volume of plasma was consistent with the concentration of 0.2 micrograms/ml measured by RIA in the nuclear extract. Total specific binding of [125I] T4 and [125I] T3 can be attributed to plasma contamination, as 0.3 microliter of plasma gave identical binding. Furthermore, a TBG antiserum abolished specific T4 and T3 binding to the nuclear protein extract. These data are consistent with TBG being the major plasma contaminant. In fourteen normal, euthyroid subjects, the higher mean maximum binding capacity (MBC) for T4 (1.63 +/- 0.27 SD) compared to T3 (0.25 +/- 0.21 pmol/mg protein; P less than 0.001) and the same binding affinity (Ka, 2.0 +/- 0.7 x 10(9)/mol) for both T4 and T3 are the same as found in plasma. The higher MBC for T4 (5.8 +/- 1.0) and T3 (0.69 +/- 0.2 pmol/mg protein) in two hypothy...Continue Reading

References

Jan 1, 1977·The Journal of Clinical Endocrinology and Metabolism·K A Woeber
Mar 1, 1978·Acta Endocrinologica·T Lemarchand-BéraudA Burger
Mar 9, 1979·Science·J H Oppenheimer
Apr 1, 1979·The Journal of Clinical Endocrinology and Metabolism·L D SchusterJ H Oppenheimer
Mar 1, 1977·Clinical Endocrinology·J Bernal, S Refetoff
Feb 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·K LiewendahlB A Lamberg
May 1, 1974·The Journal of Clinical Endocrinology and Metabolism·J S Tsai, H H Samuels
Apr 1, 1980·Endocrinology·H C SmithC J Eastman
Jul 1, 1980·The Journal of Clinical Endocrinology and Metabolism·K D BurmanL Wartofsky
Feb 1, 1982·The Australian Journal of Experimental Biology and Medical Science·G F MaberlyT J Wilke
Apr 1, 1981·The Journal of Clinical Endocrinology and Metabolism·R GärtnerP C Scriba

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Citations

Jun 1, 1987·General and Comparative Endocrinology·C C Wong, K W Chiu
Nov 1, 1985·Scandinavian Journal of Clinical and Laboratory Investigation·J BernalB A Lamberg
Jan 1, 1986·Endocrine Research·R W Evans, L E Braverman

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