Plasma-Derived Exosomal Circular RNA hsa_circ_0005540 as a Novel Diagnostic Biomarker for Coronary Artery Disease.

Disease Markers
Wei-Peng WuXing-Dong Xiong

Abstract

Exosomes exist in almost all body fluid and contain diverse biological contents which may be reflective of disease state. Circular RNAs (circRNAs) are stable in structure and have a long half-life in exosomes without degradation, thus making them reliable biomarkers. However, the potential of exosomal circRNAs as biomarkers of coronary artery disease (CAD) remains to be established. Here, we aimed to investigate the expression levels and the potential use of exosomal circRNAs as diagnostic biomarkers for CAD. CircRNA expression levels in exosomes obtained from three plasma samples of CAD patients and three paired controls were analyzed using RNA sequencing. Exosomal circRNAs obtained in the profiling phase were then verified in two-center validation cohorts. Finally, the ability of exosomal circRNAs, adjusting for Framingham Heart Study (FHS) risk factors, was determined to discriminate between CAD patients and non-CAD controls. 355 circRNAs were differentially expressed between these two groups: 164 were upregulated, and 191 were downregulated. Here, we selected the potential circRNAs (fold change > 4, P < 0.05) as candidate biomarkers for further validation. Our data showed that only hsa_circ_0005540 was significantly associa...Continue Reading

References

Jan 5, 2002·The New England Journal of Medicine·S Achenbach, W G Daniel
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Trairak PisitkunMark A Knepper
May 24, 2005·International Immunology·Marie-Pierre CabyChristian Bonnerot
Jun 17, 2005·Nephrology·Ewout J HoornMark A Knepper
Jun 6, 2009·Nature Reviews. Immunology·Clotilde ThéryElodie Segura
Jan 5, 2011·Biochemical and Biophysical Research Communications·Qiuwang ZhangMichael J Kutryk
Apr 21, 2011·Nature Communications·María MittelbrunnFrancisco Sánchez-Madrid
Apr 19, 2012·Nature Reviews. Molecular Cell Biology·Maria Mittelbrunn, Francisco Sánchez-Madrid
Aug 9, 2012·Cell Biochemistry and Biophysics·Jian Ling SunChong Yu Wang
Dec 20, 2012·RNA·William R JeckNorman E Sharpless
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Mar 1, 2013·Nature·Thomas B HansenJørgen Kjems
Mar 1, 2013·Nature·Sebastian MemczakNikolaus Rajewsky
Sep 17, 2013·Molecular Cell·Yang ZhangLing-Ling Chen
Sep 17, 2013·PLoS Genetics·Julia SalzmanPatrick O Brown
May 29, 2014·International Journal of Molecular Sciences·Hitoshi Suzuki, Toshifumi Tsukahara
Jun 7, 2014·Frontiers in Oncology·Jacob A TicknerKenneth J O'Byrne
Sep 23, 2014·Molecular Cell·Reut Ashwal-FlussSebastian Kadener
Feb 11, 2015·Nature Structural & Molecular Biology·Zhaoyong LiGe Shan
Mar 10, 2015·RNA Biology·Ling-Ling Chen, Li Yang
Mar 19, 2015·Research in Cardiovascular Medicine·Behshid GhadrdoostAta Firouzi
Apr 2, 2016·The Journal of Clinical Investigation·Raghu Kalluri
Aug 5, 2017·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Xuejie LiMuwei Li
Jan 24, 2018·Journal of Extracellular Vesicles·Christian PreußerAlbrecht Bindereif
Feb 1, 2019·Circulation·Emelia J BenjaminUNKNOWN American Heart Association Council on Epidemiology and Prevention Statistics Committee and Stroke Statistics Subcommittee

❮ Previous
Next ❯

Citations

Aug 28, 2020·Disease Markers·Christos MasaoutisStamatios Theocharis
Dec 8, 2020·Frontiers in Pharmacology·Chuan SunLu Cao
Dec 29, 2020·Frontiers in Pharmacology·Jia LiuJunnan Wang
Dec 10, 2020·International Journal of Molecular Sciences·Raquel Figuinha VideiraInês Falcão-Pires
Mar 13, 2021·Frontiers in Cardiovascular Medicine·Soudeh Ghafouri-FardMohammad Taheri

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.