Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients.

Critical Care : the Official Journal of the Critical Care Forum
Andrew RhodesE David Bennett

Abstract

Risk stratification of severely ill patients remains problematic, resulting in increased interest in potential circulating markers, such as cytokines, procalcitonin and brain natriuretic peptide. Recent reports have indicated the usefulness of plasma DNA as a prognostic marker in various disease states such as trauma, myocardial infarction and stroke. The present study assesses the significance of raised levels of plasma DNA on admission to the intensive care unit (ICU) in terms of its ability to predict disease severity or prognosis. Fifty-two consecutive patients were studied in a general ICU. Blood samples were taken on admission and were stored for further analysis. Plasma DNA levels were estimated by a PCR method using primers for the human beta-haemoglobin gene. Sixteen of the 52 patients investigated died within 3 months of sampling. Nineteen of the 52 patients developed either severe sepsis or septic shock. Plasma DNA was higher in ICU patients than in healthy controls and was also higher in patients who developed sepsis (192 (65-362) ng/ml versus 74 (46-156) ng/ml, P = 0.03) or who subsequently died either in the ICU (321 (185-430) ng/ml versus 71 (46-113) ng/ml, P < 0.001) or in hospital (260 (151-380) ng/ml versus 68...Continue Reading

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Dec 1, 2011·Biopreservation and Biobanking·Joseph V Geisberg, Todd Basque
Aug 16, 2011·Critical Care : the Official Journal of the Critical Care Forum·Marjatta OkkonenUNKNOWN FINNALI Study Group
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Methods Mentioned

BETA
PCR

Software Mentioned

Lightcycler

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