Plasma Exosomal miR-450b-5p as a Possible Biomarker and Therapeutic Target for Transient Ischaemic Attacks in Rats

Journal of Molecular Neuroscience : MN
Xiu-Mei LuoJingli Liu

Abstract

Transient ischaemic attack (TIA) and cerebral infarction are difficult to identify within the thrombolytic time window. Blood markers are efficient, economical and noninvasive and can be beneficial in the diagnosis of many diseases. Plasma exosomal biomarkers are rarely reported in TIA. Exosomal microRNAs (miRNAs) were extracted from plasma and cerebrospinal fluid after middle cerebral artery occlusion (MCAo) in rats (0 min, 5 min, 10 min, 2 h). Deep sequencing was used to detect exosomal miRNAs in rat plasma and confirm significant differentially expressed miRNAs. Polymerase chain reaction (PCR) was used to detect the differentially expressed miRNAs in plasma and cerebrospinal fluid. Exosomal miRNAs with the same expression trends in plasma and cerebrospinal fluid were selected, and bioinformatics analysis was then carried out. Finally, the area under the curve (AUC) of the receiver operating characteristic (ROC) curve was determined to assess the diagnostic accuracy of miRNAs for TIA in rats. First, high-throughput sequencing was used to detect the expression level of plasma exosome miRNA, and rno-miR-450b-5p with a decreasing expression level was screened. Second, the expression levels of exosomal miRNAs were verified in cer...Continue Reading

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Citations

Jan 17, 2021·Molecular Diagnosis & Therapy·Ghada YousifAshfaq Shuaib
Feb 3, 2021·Molecular and Cellular Biochemistry·Yifei YuGuangfan Chi
May 14, 2021·Translational Stroke Research·Yiyang LiYonghua Zhao
Jul 11, 2021·Journal of Neurointerventional Surgery·Jeong-Min KimHye Ryoun Kim

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Methods Mentioned

BETA
PCR
transmission electron microscopy
electrophoresis
biopsy

Software Mentioned

QuantaSoft
StepOne
TargetScan
SPSS
miRWalk
edge R

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