Plasma exosomes characterization reveals a perioperative protein signature in older patients undergoing different types of on-pump cardiac surgery.

GeroScience
Alessandro CarrozzoVincenzo Lionetti

Abstract

Although exosomes are extracellular nanovesicles mainly involved in cardioprotection, it is not known whether plasma exosomes of older patients undergoing different types of on-pump cardiac surgery protect cardiomyocytes from apoptosis. Since different exosomal proteins confer pro-survival effects, we have analyzed the protein cargo of exosomes circulating early after aortic unclamping. Plasma exosomes and serum cardiac troponin I levels were measured in older cardiac surgery patients (NYHA II-III) who underwent first-time on-pump coronary artery bypass graft (CABG; n = 15) or minimally invasive heart valve surgery (mitral valve repair, n = 15; aortic valve replacement, n = 15) at induction of anesthesia (T0, baseline), 3 h (T1) and 72 h (T2) after aortic unclamping. Anti-apoptotic role of exosomes was assessed in HL-1 cardiomyocytes exposed to hypoxia/re-oxygenation (H/R) by TUNEL assay. Protein exosomal cargo was characterized by mass spectrometry approach. Exosome levels increased at T1 (P < 0.01) in accord with troponin values in all groups. In CABG group, plasma exosomes further increased at T2 (P < 0.01) whereas troponin levels decreased. In vitro, all T1-exosomes prevented H/R-induced apoptosis. A total of 340 exosomal p...Continue Reading

References

Mar 12, 1999·Journal of Molecular and Cellular Cardiology·P AbeteG Ambrosio
Nov 1, 2002·The Journal of Biological Chemistry·Atsushi SuzukiHiroyasu Esumi
Oct 26, 2005·The Annals of Thoracic Surgery·Domenico PaparellaLuigi de Luca Tupputi Schinosa
Jul 31, 2008·Critical Care Medicine·Moritoki EgiMichael Bailey
Jan 30, 2009·Cardiovascular Research·Kerstin BoenglerGerd Heusch
Mar 6, 2009·American Journal of Physiology. Cell Physiology·Ann-Dorit AndersenStine Falsig Pedersen
Jun 17, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Pierluigi Mauri, Michaela Scigelova
Jan 27, 2010·Journal of Cellular and Molecular Medicine·Francesca ForiniGiorgio Iervasi
Jul 6, 2011·Journal of Molecular Biology·John F AndersonJosé M Barral
Feb 5, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Z A MalikA A Knowlton
Jun 1, 2013·Cardiovascular Research·Hannah J WhittingtonDerek M Yellon
Jan 22, 2014·Journal of Cellular and Molecular Medicine·Vincenzo LionettiAngela Pucci
Feb 7, 2014·Cardiovascular Diabetology·Mariëtte E G KranendonkUNKNOWN SMART Study Group
Apr 29, 2014·Seminars in Cancer Biology·Carolina Villarroya-BeltriMaría Mittelbrunn
May 9, 2014·BioMed Research International·Francesco NicoliniTiziano Gherli
Jun 22, 2014·Journal of Immunological Methods·Laurent MullerTheresa L Whiteside
Jul 16, 2014·Journal of Cardiovascular Medicine·Florinda MastroDomenico Paparella
Mar 13, 2015·Frontiers in Immunology·Matías Sáenz-CuestaDavid Otaegui
Apr 18, 2015·Journal of the American College of Cardiology·Jose M VicencioSean M Davidson
Apr 30, 2015·International Journal of Molecular Sciences·Beatriz García-FontanaManuel Muñoz-Torres
May 15, 2015·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Ashima SinghUNKNOWN BARI 2D Study Group
May 28, 2016·Nature Reviews. Endocrinology·Adeel SafdarMark A Tarnopolsky
Jul 26, 2016·International Journal of Molecular Sciences·Ainhoa AlberroDavid Otaegui
Sep 10, 2016·Journal of the American Heart Association·Daniele PastoriFrancesco Violi
Sep 28, 2016·Journal of Cardiothoracic and Vascular Anesthesia·Vladimir V LomivorotovAlexander M Karaskov
Mar 23, 2017·Critical Care Medicine·Nora Terrasini, Vincenzo Lionetti
May 10, 2017·EURASIP Journal on Bioinformatics & Systems Biology·Danila VellaDario Di Silvestre
Mar 13, 2018·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Sarinnapha M VasunilashornEdward R Marcantonio
May 4, 2018·Diabetes·David W FreemanMichele K Evans

❮ Previous
Next ❯

Citations

Oct 8, 2020·Molecular Biology Reports·Fatemeh Javaherforoosh ZadehHadi Rezaeeyan
Nov 24, 2020·The International Journal of Biochemistry & Cell Biology·Hao LingChunli Song
Oct 2, 2020·Canadian Journal of Physiology and Pharmacology·Carlotta Baroni, Vincenzo Lionetti
Mar 31, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Vincenzo LionettiTommaso Angelone
Aug 8, 2021·International Journal of Molecular Sciences·Marta ZaràSilvia S Barbieri
Aug 8, 2021·International Journal of Molecular Sciences·Ainhoa AlberroDavid Otaegui

❮ Previous
Next ❯

Software Mentioned

Thermo
Scientific Proteome Discoverer
STRING
Sequest HT
Matlab Statistics Machine Learning Toolbox
BINGO
Cytoscape

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Clinical Chemistry and Laboratory Medicine : CCLM
Huan YangXu Zhang
Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
James D Perkins
Nature Biotechnology
© 2021 Meta ULC. All rights reserved