Abstract
Endometrial cancer (EC) is a common gynaecological cancer worldwide. Exosomes, secreted by living cells and detected in various body fluids, can exchange information between organs and compartments to affect cellular functions, such as proliferation, apoptosis, migration and angiogenesis. We hypothesise that plasma exosomal contents are altered during cancer progression and promote cancer growth and angiogenesis by delivering biomolecules to cancer and vascular endothelial cells. In this study, circulating exosomes derived from EC patients and age-matched healthy people were acquired by commercial kits. Cell counting kit-8, Transwell and Matrigel tube formation assays showed that circulating exosomes from EC patients promote EC cell growth and human umbilical vein endothelial cell (HUVEC) angiogenesis. Next, proteomic analysis and ELISA revealed that plasma exosomal lectin galactoside-binding soluble 3 binding protein (LGALS3BP) increased during EC progression. Moreover, to explore the function of exosomal LGALS3BP, we acquired exosomes containing high levels of LGALS3BP by overexpressing LGALS3BP in human embryonic kidney 293 cells, and we demonstrated that highly contained exosomal LGALS3BP contributed to EC cell proliferatio...Continue Reading
References
Jan 1, 1994·Trends in Biochemical Sciences·D ResnickM Krieger
Nov 6, 2001·The Journal of Biological Chemistry·Zhen Y JiangMichael P Czech
Aug 3, 2002·Nature Reviews. Immunology·Clotilde ThérySebastian Amigorena
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Trairak PisitkunMark A Knepper
Sep 3, 2004·Molecular Biology of the Cell·Nabendu PoreAmit Maity
May 24, 2005·International Immunology·Marie-Pierre CabyChristian Bonnerot
Nov 17, 2005·Oncogene·Deborah A Altomare, Joseph R Testa
Feb 28, 2006·Kidney International·H ZhouR A Star
Oct 26, 2011·The International Journal of Biochemistry & Cell Biology·Anna-Kristin Ludwig, Bernd Giebel
May 24, 2012·Nucleic Acids Research·Jessica WahlgrenHadi Valadi
Aug 7, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Enza PiccoloStefano Iacobelli
Mar 5, 2013·The Journal of Clinical Investigation·Sampurna ChatterjeeRoland T Ullrich
May 4, 2013·Carcinogenesis·Kartick C PramanikSanjay K Srivastava
Dec 24, 2013·Oncogene·P StampolidisS Iacobelli
May 23, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sui PengMing Kuang
Jun 25, 2015·Nature·Sonia A MeloRaghu Kalluri
Nov 3, 2015·Nature·Ayuko HoshinoDavid Lyden
Feb 18, 2017·Nature Communications·Yun TengHuang-Ge Zhang
Jul 15, 2017·Cell·Irina MateiDavid Lyden
Jul 22, 2017·Neoplasia : an International Journal for Oncology Research·Darshan S ChandrashekarSooryanarayana Varambally
Nov 17, 2017·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·M WangA Wu
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Feb 6, 2018·Cancer Biomarkers : Section a of Disease Markers·Jianjun WangGuangxin Li
Feb 14, 2018·Cancer Research·Divya BhagirathSharanjot Saini
Aug 15, 2018·Molecular Oncology·Chi PanHeidi Schwarzenbach
Sep 13, 2018·CA: a Cancer Journal for Clinicians·Freddie BrayAhmedin Jemal
Dec 27, 2018·Journal of Proteome Research·Javier MariscalMiguel Abal
Jul 14, 2019·Brain Research·Yan JiangXingyuan Sun
Jul 16, 2019·Cellular Signalling·Xiaoxin ZhangYanhong Ni
May 28, 2020·Signal Transduction and Targeted Therapy·Xiaoli WenXiaoping Wan
Citations
Mar 7, 2021·International Journal of Molecular Sciences·Sahar EsfandyariAyman Al-Hendy
Jul 25, 2021·Cancers·Blendi UraGiuseppe Ricci
Sep 28, 2021·Journal of Translational Medicine·Emily CaponeGianluca Sala
Oct 29, 2021·Journal of Biomedical Nanotechnology·Lijuan HuangLiang Zhao
Oct 19, 2021·Frontiers in Cell and Developmental Biology·Hyobin Julianne LimDae-Kyum Kim