PMID: 8963720May 1, 1996Paper

Plasma fibrinogen inhibits platelets adhesion in flowing blood to immobilized fibrinogen

Arteriosclerosis, Thrombosis, and Vascular Biology
S C EndenburgPhilip G de Groot

Abstract

The influence of variations in plasma fibrinogen concentration on platelet adhesion to immobilized fibrinogen was investigated in a parallel-plate perfusion chamber. At a shear rate of 1600 s-1 platelet adhesion decreased when increasing concentrations of purified fibrinogen were added to the plasma (IC50 = 1.5 +/- 0.2 g/L fibrinogen, n = 24). Washed platelets reconstituted in a human albumin solution with red blood cells were more sensitive for soluble fibrinogen (IC50 = 0.4 +/- 0.1 g/L, n = 5, P < .05). When platelet activation during circulation of the blood was minimized by using a single-passage perfusion system, an IC50 of 2.0 +/- 0.2 g/L was found (n = 9). To exclude the possibility that the inhibition of fibrinogen was caused by irreversible changes in the fibrinogen molecule during the purification procedure, normal plasma was mixed in different ratios with plasma from a patient with congenital afibrinogenemia. Under these conditions, the plasma fibrinogen IC50 was 1.5 +/- 1.1 g/L. Absence of endogenous fibrinogen in the platelets of the patient resulted in an IC50 of 1.2 +/- 0.5 g/L for plasma fibrinogen. These results demonstrate that increased plasma fibrinogen concentrations inhibit platelet adhesion to fibrinogen ...Continue Reading

References

Nov 1, 1979·The Journal of Clinical Investigation·J S Bennett, G Vilaire
Sep 14, 1991·BMJ : British Medical Journal·E Ernst
Nov 1, 1991·The American Journal of Physiology·C J Jen, J S Lin
Mar 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·E B SmithC Stirk
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·A BiniK L Kaplan
Aug 1, 1986·Clinical Science·G D Lowe
Aug 15, 1986·European Journal of Biochemistry·G CourtoisG A Marguerie
Jun 1, 1985·Thrombosis Research·T W MeadeM J Seghatchian
Jun 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·M B Zucker, L Vroman
Feb 1, 1982·The Journal of Clinical Investigation·R L Nachman, L L Leung
Dec 1, 1995·The Journal of Biological Chemistry·B SavageZ M Ruggeri
Jan 1, 1994·The Journal of Clinical Investigation·J J ZwagingaT J Rabelink
Feb 1, 1993·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·S C EndenburgJ J Zwaginga

❮ Previous
Next ❯

Citations

Oct 23, 2012·The Journal of Biological Chemistry·Ivan S YermolenkoTatiana P Ugarova
Aug 17, 2002·Journal of Biomaterials Science. Polymer Edition·V Balasubramanian, S M Slack
Jul 17, 2014·Nature Communications·Susanne M de WittJudith M E M Cosemans
May 11, 2002·Photochemistry and Photobiology·Patrick FungaloiGlenn LaMuraglia
Jan 26, 2010·Journal of Thrombosis and Haemostasis : JTH·V K LishkoT P Ugarova
Dec 31, 2019·Journal of Thrombosis and Haemostasis : JTH·Toshko LissitchkovFlora Peyvandi
Jan 15, 2021·International Journal of Molecular Sciences·Richard J FishMarguerite Neerman-Arbez
May 29, 2000·Biophysical Journal·A BonnefoyM M Frojmovic

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.