Plasma free DNA: Evaluation of temperature-associated storage effects observed for Roche Cell-Free DNA collection tubes

Biochemia Medica
Dietmar EnkoGernot Kriegshäuser

Abstract

Standardized pre-analytical blood sample procedures for the analysis of circulating cell-free DNA (ccfDNA) are still not available. Therefore, the present study aimed at evaluating the impact of storage conditions related to different times (24 and 48 h) and temperatures (room temperature (RT) and 4 - 8 °C) on the plasma ccfDNA concentration of blood samples drawn into Cell-Free DNA collection tubes (Roche Diagnostics GmbH, Mannheim, Germany). Venous blood from 30 healthy individuals was collected into five 8.5 mL Cell-Free DNA Collection Tubes (Roche Diagnostics GmbH) each. Plasma samples were processed at time point of blood collection (tube 1), and after storage under the following conditions: 24 h at RT (tube 2) or 4-8 °C (tube 3), and 48 h at RT (tube 4) or 4 - 8 °C (tube 5). Circulating cell-free DNA concentrations were determined by EvaGreen chemistry-based droplet digital PCR (ddPCR). No statistically significant differences between median (interquartile range) plasma ccfDNA concentrations (ng/mL) at time point of blood collection (3.17 (2.13 - 3.76)) and after storage for 24 h (RT: 3.02 (2.41 - 3.68); 4-8 °C: 3.21 (2.19 - 3.46)) and 48 h (RT: 3.13 (2.10 - 3.76); 4-8 °C: 3.09 (2.19 - 3.50)) were observed (P values from ...Continue Reading

References

Jun 4, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Safia El MessaoudiAlain R Thierry
Jul 10, 2013·Nature Reviews. Clinical Oncology·Emily CrowleyAlberto Bardelli
Apr 5, 2017·Clinica Chimica Acta; International Journal of Clinical Chemistry·Bernd SchmidtMichael Fleischhacker
Nov 16, 2017·Analytical Biochemistry·Sergey NikolaevThierry Nouspikel

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Citations

May 13, 2020·Critical Reviews in Clinical Laboratory Sciences·Vida UngererStefan Holdenrieder
Dec 16, 2019·Molecular Diagnosis & Therapy·Laure SorberPatrick Pauwels
Dec 4, 2020·Expert Review of Molecular Diagnostics·O E BryzgunovaP P Laktionov
Nov 20, 2020·International Journal of Molecular Sciences·Zuzana PösTomas Szemes
Sep 23, 2021·Fetal and Pediatric Pathology·Jianglan LuoGuanhua Deng

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Methods Mentioned

BETA
blood collection
blood drawn
DNA
PCR
electrophoresis
blood drawing
DNA collection

Software Mentioned

Bio
Rad QuantaSoft
Analyse
- it

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