Plasma haptoglobin and immunoglobulins as diagnostic indicators of deoxynivalenol intoxication.

Journal of Veterinary Science
Eun Joo KimKyoung Ho Choi

Abstract

This study aimed to discover potential biomarkers for dioxynivalenol (DON) intoxication. B6C3F1 male mice were orally exposed to 0.83, 2.5 and 7.5 mg/kg body weight (bw) DON for 8 days and the differential protein expressions in their blood plasma were determined by SELDI - Time-of-Flight/Mass Spectrometry (TOF/MS) and the immunoglobulins (Igs) G, A, M and E in the serum were investigated. 11.7 kDa protein was significantly highly expressed according to DON administration and this protein was purified by employing a methyl ceramic HyperD F column with using optimization buffer for adsorption and desorption. The purified protein was identified as a haptoglobin precursor by peptide mapping with using LC/Q-TOF/MS and MALDI-TOF/MS and this was confirmed by western blotting and ELISA. IgG and IgM in serum were decreased in a dose-dependent manner and IgA was decreased at 7.5 mg/kg bw DON administration, but the IgE level was not changed. To compare the expressions of haptoglobin and the Igs patterns between aflatoxin B1 (AFB1), zearalenone (ZEA) and DON intoxications, rats were orally administered with AFB1 1.0, ZEA 240 and DON 7.5 mg/kg bw for 8 days. Haptoglobin was increased only at DON 7.5 mg/kg bw, while it was slightly decreas...Continue Reading

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Jun 26, 2013·Toxicology Letters·Mohammad Rafiqul IslamBumseok Kim
May 31, 2012·Archives of Animal Nutrition·Annabella Khol-ParisiniJürgen Zenteke
Jan 13, 2011·The Journal of Veterinary Medical Science·Osamu MikamiKen Katsuda
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May 21, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kee Hun DoYuseok Moon

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Methods Mentioned

BETA
chip
protein chip
chips
ELISA

Software Mentioned

Mascot
ProFound

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