Plasma HPV cell-free DNA monitoring in advanced HPV-associated oropharyngeal cancer

Annals of Oncology : Official Journal of the European Society for Medical Oncology
Glenn J HannaCloud P Paweletz

Abstract

Measuring cell-free (cf)DNA in blood and tissues holds significant potential as a minimally invasive method for disease monitoring in cancer. Cancers arising in the oropharynx and causally linked to human papillomavirus (HPV) represent an ideal model in which to interrogate these methods. We designed an ultrasensitive and quantitative droplet digital (dd)PCR assay to detect the five dominant high-risk HPV subtypes linked to oropharyngeal cancer (OPC). We enrolled a pilot observational cohort of 22 patients with advanced HPV+ OPC to evaluate the clinical utility of our assay and explore its predictive and prognostic potential. Total tumor burden (TTB) strongly correlated with HPV cfDNA levels (R = 0.91, P = 2.3×10-6) at this cohort size, and in most cases more distant anatomic disease locations predicted increasing HPV cfDNA levels. All participants demonstrated a corresponding change in their HPV cfDNA levels at a median of 16 days (range 12-38) before restaging scans confirming treatment response or progression. Patients with locoregional disease in the head and neck or pulmonary-only metastases had worse outcomes (P = 0.01). Both TTB and median plasma HPV cfDNA levels negatively correlated with survival (R=-0.65, P = 0.01; R=...Continue Reading

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Citations

May 6, 2019·Human Vaccines & Immunotherapeutics·Mary Roz TimbangMichael G Moore
Jul 18, 2019·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·K ParameshwaranW D Rawlinson
Sep 9, 2020·International Journal of Cancer. Journal International Du Cancer·Hidenori TanakaHidenori Inohara
Feb 6, 2020·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Bhishamjit S CheraGaorav P Gupta
Sep 27, 2019·Frontiers in Oncology·Panagiota EconomopoulouAmanda Psyrri
Jun 21, 2020·Scientific Reports·Ana Carolina de CarvalhoAndré Lopes Carvalho
Apr 4, 2019·Molecular Diagnosis & Therapy·Re-I ChinAadel A Chaudhuri
May 22, 2020·Frontiers in Oncology·Petr SzturzJan B Vermorken
Nov 10, 2020·CA: a Cancer Journal for Clinicians·Michael L ChengGeoffrey R Oxnard
Feb 10, 2021·Current Treatment Options in Oncology·Sarah M DermodyPaul L Swiecicki
Feb 15, 2021·Oral Oncology·Kate Chatfield-ReedQuintin Pan
Jul 1, 2021·Hematology/oncology Clinics of North America·Harish N Vasudevan, Sue S Yom
Jul 11, 2021·Hematology/oncology Clinics of North America·Eleni M Rettig, Rosh K V Sethi
Sep 29, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Eric LeungScott V Bratman
Jul 3, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Emmanuelle JeannotSuzy Scholl
Aug 22, 2020·The Journal of Molecular Diagnostics : JMD·Henrike RederSteffen Wagner
Nov 14, 2021·Molecular Diagnosis & Therapy·Susana Olmedillas-LópezDamián García-Olmo
Oct 28, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yue CaoJ Chad Brenner

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