Plasma levels of carboxypeptidase U (CPU, CPB2 or TAFIa) are elevated in patients with acute myocardial infarction

Journal of Thrombosis and Haemostasis : JTH
D LeenaertsDirk Hendriks

Abstract

Two decades after its discovery, carboxypeptidase U (CPU, CPB2 or TAFIa) has become a compelling drug target in thrombosis research. However, given the difficulty of measuring CPU in the blood circulation and the demanding sample collecton requirements, previous clinical studies focused mainly on measuring its inactive precursor, proCPU (proCPB2 or TAFI). Using a sensitive and specific enzymatic assay, we investigated plasma CPU levels in patients presenting with acute myocardial infarction (AMI) and in controls. In this case-control study, peripheral arterial blood samples were collected from 45 patients with AMI (25 with ST segment elevation myocardial infarction [STEMI], 20 with non-ST segment elevation myocardial infarction [NSTEMI]) and 42 controls. Additionally, intracoronary blood samples were collected from 11 STEMI patients during thrombus aspiration. Subsequently, proCPU and CPU plasma concentrations in all samples were measured by means of an activity-based assay, using Bz-o-cyano-Phe-Arg as a selective substrate. CPU activity levels were higher in patients with AMI (median LOD-LOQ, range 0-1277 mU L(-1) ) than in controls (median < LOD, range 0-128 mU L(-1) ). No correlation was found between CPU levels and AMI type...Continue Reading

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Citations

Mar 11, 2017·Cardiovascular Diabetology·Katherine KearneyRamzi Ajjan
Dec 29, 2019·Biomolecules·Meenashi Vanathi BalashanmugamChanabasayya Vastrad
Jan 31, 2017·Journal of Thrombosis and Haemostasis : JTH·A-C Jönsson RylanderK Wåhlander
Jan 23, 2021·International Journal of Molecular Sciences·Karen ClaesenDirk Hendriks
Jun 18, 2021·Hämostaseologie·Abdulkerim YıldızMurat Albayrak

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