Plasma levels of vitellogenin in Chrysemys picta during the annual gonadal cycle: measurement by specific radioimmunoassay

Endocrinology
D A GappI P Callard

Abstract

A RIA for turtle (Chrysemys picta) vitellogenin is described. After dimethylformamide precipitation of vitellogenin from the plasma of estrogen-treated female turtles, antibodies were developed in rabbits. The dimethylformamide precipitate was further purified by o-triethylaminoethyl cellulose column chromatography; the vitellogenin component eluted as a single peak. This material was used for iodination by a mild chloramine method. Antibodies to turtle vitellogenin did not cross-react with plasma from male turtles or vitellogenic females of other vertebrate groups, including lizards and snakes. Limited cross-reactivity exists among the chelonians, however. Using a 1:5000 dilution of antiserum, the limit of detection was 15 ng, and the midrange was 320 +/- 45 ng. For an antiserum dilution of 1:1000, these figures were 30 and 600 +/- 37 ng, respectively. Using this assay, the seasonal pattern of plasma vitellogenin in the turtle has been described, and preliminary studies on in vitro hepatic vitellogenesis have been performed.

Citations

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Related Concepts

Cross Reactions
Estradiol, (16 alpha,17 beta)-Isomer
Immunodiffusion Measurement
Iodine Radioisotopes
Isotope Labeling, Stable
Lipoproteins
Liver
Radioimmunoassay
Seasonal Variation
Turtle

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