PMID: 6988626Apr 1, 1980Paper

Plasma membrane and intracellular lipid synthesis in tumor cells rendered resistant to humoral immune killing after treatment with hormones

Journal of the National Cancer Institute
S I Schlager, S H Ohanian

Abstract

Line-10 hepatoma cells from Sewall Wright guinea pigs are sensitive to killing by antibody plus human complement. Hormones that decrease the sensitivity of the cells to antibody-complement-mediated killing (insulin and hydrocortisone) were examined for their effects on the ability of the cells to synthesize and incorporate specific lipids into plasma membrane and intracellular membrane fractions. Cells that had been rendered resistant to antibody-complement-mediated killing following incubation for 1 hour with either of the hormones were enhanced in their incorporation of newly synthesized L-alpha-phosphatidyl serine, L-alpha-phosphatidyl choline, and triglycerides into the plasma membrane as well as L-alpha-phosphatidyl choline, L-alpha-phosphatidyl serine, and cholesteryl ester into mitochondria, endoplasmic reticulum, nuclear membrane, or microsomes; these cells were inhibited in cardiolipin synthesis. Cells cultured for 4 hours with hormone regained their sensitivity to antibody-complement-mediated killing and reverted to control levels in their ability to synthesize and incorporate lipids into plasma and intracellular membranes. These data suggest that agents that increase the resistance of the tumor cells to humoral immun...Continue Reading

Related Concepts

Tumor Antibodies
Plasma Membrane
Epicholesterol
Hemolytic Complement
Immunologic Tumoricidal Activities
Cavia porcellus
Hydrocortisone, (9 beta,10 alpha,11 alpha)-Isomer
Novolin
Intracellular Membranes
Hepatoma, Novikoff

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