Plasma membrane-associated glycohydrolases along differentiation of murine neural stem cells.

Neurochemical Research
Massimo AureliSandro Sonnino

Abstract

The activities of plasma membrane associated sialidase Neu3, total β-glucosidase, CBE-sensitive β-glucosidase, non-lysosomal β-glucosyl ceramidase GBA2, β-galactosidase, β-hexosaminidase and sphingomyelinase were determined at three different stages of differentiation of murine neural stem cell cultures, corresponding to precursors, commited progenitors, and differentiated cells. Cell immunostaining for specific markers of the differentiation process, performed after 7 days in culture in presence of differentiating agents, clearly showed the presence of oligodendrocytes, astrocytes and neurons. Glial cells were the most abundant. Sialidase Neu3 after a decrease from progenitors to precursors, showed an increase parallel to the differentiation process. All the other glycosidases increased their activity along differentiation. The activity of CBE-sensitive β-glucosidase and GBA2 were very similar at the precursor stage, but CBE-sensitive β-glucosidase increased 7 times while GBA2 only two in the differentiated cells. In addition, we analysed also sphingomyelinase as enzyme specifically associated to sphingolipids. The activity of this enzyme increased from precursors to differentiated cells.

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Citations

Dec 24, 2013·Molecular Neurobiology·Sandro SonninoAlessandro Prinetti
Sep 24, 2013·Biochemical and Biophysical Research Communications·Alessandro MaginiCarla Emiliani
Jan 22, 2013·American Journal of Human Genetics·Monia B HammerAndrew B Singleton
Dec 14, 2017·Frontiers in Molecular Neuroscience·Marina A Woeste, Dagmar Wachten
Nov 28, 2013·Molecular Neurobiology·Massimo AureliSandro Sonnino
Aug 6, 2015·Expert Opinion on Therapeutic Targets·Wei-Yi OngAkhlaq A Farooqui
May 11, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maura SamaraniSandro Sonnino

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