Plasma membrane-associated glycohydrolases activation by extracellular acidification due to proton exchangers.

Neurochemical Research
Massimo AureliAlessandro Prinetti

Abstract

In this paper, we show that the pH optimum for the plasma membrane (PM)-associated activity of four glycohydrolases (conduritol B epoxide sensitive β-glucosidase, β-glucosidase GBA2, β-hexosaminidase and β-galactosidase) measured on intact cells is acidic. Moreover, we show that drugs able to modify the efflux of protons across the PM, thus locally affecting the extracellular proton concentration close to the PM, are able to modulate the activities of these enzymes. These data strongly suggest that pH-dependent modulation of PM-associated glycohydrolases activities could be an effective way to locally modulate the cell surface glycoconjugate composition.

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Citations

Dec 24, 2013·Molecular Neurobiology·Sandro SonninoAlessandro Prinetti
Nov 26, 2015·Neurochemical Research·Aureli MassimoSonnino Sandro
Jul 1, 2018·Journal of Neurochemistry·Sara GrassiAlessandro Prinetti
Oct 13, 2018·International Journal of Molecular Sciences·Anna MalekkouKyproula Christodoulou
Aug 27, 2018·Glycoconjugate Journal·Massimo AureliSandro Sonnino
Jun 23, 2019·Biochimie·Dominique Lagadic-GossmannLaurence Huc
May 19, 2021·FEBS Open Bio·Giulia LunghiSandro Sonnino

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