Plasma membrane Ca2+ ATPase isoform 4b binds to membrane-associated guanylate kinase (MAGUK) proteins via their PDZ (PSD-95/Dlg/ZO-1) domains.

The Journal of Biological Chemistry
E KimEmanuel E Strehler

Abstract

Plasma membrane Ca2+ ATPases are P-type pumps important for intracellular Ca2+ homeostasis. The extreme C termini of alternatively spliced "b"-type Ca2+ pump isoforms resemble those of K+ channels and N-methyl-D-aspartate receptor subunits that interact with channel-clustering proteins of the membrane-associated guanylate kinase (MAGUK) family via PDZ domains. Yeast two-hybrid assays demonstrated strong interaction of Ca2+ pump 4b with the PDZ1 + 2 domains of several mammalian MAGUKs. Pump 4b and PSD-95 could be co-immunoprecipitated from COS-7 cells overexpressing these proteins. Surface plasmon resonance revealed that a C-terminal pump 4b peptide interacted with the PDZ1 + 2 domains of hDlg with nanomolar affinity (KD = 1.6 nM), whereas binding to PDZ3 was in the micromolar range (KD = 1.2 microM). In contrast, the corresponding C-terminal peptide of Ca2+ pump 2b interacted weakly with PDZ1 + 2 and not at all with PDZ3 of hDlg. Ca2+ pump 4b bound strongly to PDZ1 + 2 + 3 of hDlg on filter assays, whereas isoform 2b bound weakly, and the splice variants 2a and 4a failed to bind. Together, these data demonstrate a direct physical binding of Ca2+ pump isoform 4b to MAGUKs via their PDZ domains and reveal a novel role of alternat...Continue Reading

References

Aug 1, 1992·The Biochemical Journal·H P AdamoJ T Penniston
Dec 11, 1992·Brain Research. Molecular Brain Research·W L StahlW R Anderson
Feb 1, 1991·The Journal of Membrane Biology·E E Strehler
Jan 1, 1991·Physiological Reviews·E Carafoli
Jan 1, 1987·Annual Review of Biochemistry·E Carafoli
Feb 1, 1995·Brain Research. Molecular Brain Research·D A ZachariasE E Strehler
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·R A LueA H Chishti
Oct 1, 1995·Current Opinion in Cell Biology·S K Kim
Feb 16, 1996·The Journal of Biological Chemistry·A K VermaJ T Penniston
Dec 1, 1995·Cell Calcium·G R Monteith, B D Roufogalis
Aug 15, 1996·Nature·J H Morais CabralR C Liddington
Oct 1, 1996·Current Opinion in Neurobiology·M Sheng, E Kim
Nov 1, 1996·Current Biology : CB·A S Fanning, J M Anderson
Oct 1, 1996·Glycobiology·P C Brandt, T C Vanaman
Dec 1, 1996·Trends in Biochemical Sciences·J Saras, C H Heldin
Feb 14, 1997·Brain Research·T P StaufferE Carafoli
Nov 1, 1996·Trends in Cell Biology·C C Garner, S Kindler

❮ Previous
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Citations

Dec 28, 1999·Journal of Molecular Recognition : JMR·D G Myszka
Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S D DimitratosP J Bryant
Dec 7, 2007·Histochemistry and Cell Biology·Beate WilhelmGerhard Aumüller
Jan 30, 2008·Pflügers Archiv : European journal of physiology·Elizabeth J CartwrightLudwig Neyses
Jun 13, 2008·Pflügers Archiv : European journal of physiology·Marisa Brini
Dec 31, 2003·Journal of Molecular Biology·Helena Sterlinko GrmJohn Doorbar
Jun 11, 2005·Journal of Molecular and Cellular Cardiology·Elizabeth J CartwrightLudwig Neyses
Feb 5, 2003·Progress in Biophysics and Molecular Biology·M CereijidoM R García-Villegas
Oct 24, 2003·Cell Calcium·Jason I E BruceDavid I Yule
Aug 6, 1999·Biochimica Et Biophysica Acta·D A Zacharias, C Kappen
Aug 15, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Yang Z HuangLin Mei
Sep 8, 1998·Current Opinion in Genetics & Development·S TsunodaC S Zuker
Aug 28, 1999·Journal of Pharmacological and Toxicological Methods·G R MonteithB D Roufogalis
Jan 21, 2009·Nature Reviews. Neuroscience·Wei Feng, Mingjie Zhang
Jul 22, 1999·Annals of the New York Academy of Sciences·M Sheng, D T Pak
Nov 28, 2007·Molecular and Cellular Biology·Martin BaumgartnerKarin Moelling
Jan 19, 1999·Annual Review of Cell and Developmental Biology·B R Stevenson, B H Keon
Apr 3, 2001·Annual Review of Neuroscience·M Sheng, C Sala
Aug 7, 2007·BMC Evolutionary Biology·Aartjan J W te VelthuisChristoph P Bagowski
Jun 27, 2006·The AAPS Journal·Andrea PiserchioDale F Mierke
May 4, 2011·World Journal of Biological Chemistry·Mary Louisa HoltonAngel L Armesilla
May 4, 2011·World Journal of Biological Chemistry·William L Dean
May 4, 2011·World Journal of Biological Chemistry·Asma Zaidi
May 4, 2011·World Journal of Biological Chemistry·Emanuel E Strehler
Dec 24, 2014·Biochimica Et Biophysica Acta·Joachim Krebs
Jun 27, 2012·Biochemical and Biophysical Research Communications·Elizabeth J Salm, Stanley A Thayer
Aug 23, 2008·Prostaglandins & Other Lipid Mediators·Vanessa H OliveiraJulio A Mignaco
May 24, 2003·Annals of the New York Academy of Sciences·Geoffrey M GoellnerEmanuel E Strehler
Apr 21, 2007·Annals of the New York Academy of Sciences·Katalin PásztyAgnes Enyedi
Apr 21, 2007·Annals of the New York Academy of Sciences·Teuta DomiMarisa Brini
Apr 21, 2007·Annals of the New York Academy of Sciences·Abdul M Ruknudin, Edward G Lakatta
Apr 21, 2007·Annals of the New York Academy of Sciences·Elizabeth J CartwrightLudwig Neyses
Apr 21, 2007·Annals of the New York Academy of Sciences·Vikram PrasadGary E Shull

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