PMID: 6163856Jan 30, 1981Paper

Plasma membrane Ca2+ transport: antagonism by several potential inhibitors

The Journal of Membrane Biology
T R HindsF F Vincenzi

Abstract

Inside-out vesicles prepared from human red blood cells took up Ca2+ by an active transport process. Membranes from the same red blood cells displayed Ca2+-activated, Mg2+-dependent adenosine triphosphatase activity. Both the initial rate of Ca2+ transport and the (Ca2+ + Mg2+)-adenosine triphosphatase activity were increased approximately twofold by the calcium binding protein, calmodulin. Activities in the absence of added calmodulin were termed basal activities. Calmodulin-activated Ca2+ transport and adenosine triphosphatase activities could be antagonized in a relatively selective fashion by the phenothiazine tranquilizer drug, trifluoperazine. High concentrations of trifluoperazine also inhibited basal Ca2+ transport and adenosine triphosphatase activity. By contrast, calmodulin binding protein from beef brain selectively antagonized the effect of calmodulin on Ca2+ transport with no inhibition of basal activity. Ruthenium red antagonized calmodulin-activated and basal activity with equal potency. The results demonstrate that although phenothiazines can act as relatively selective antagonists of calmodulin-induced effects, other effects are possible and cannot be ignored. Calmodulin-binding protein may be a useful tool in...Continue Reading

References

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Apr 15, 1979·Archives of Biochemistry and Biophysics·T J Lynch, W Y Cheung
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Citations

Feb 1, 1983·Naunyn-Schmiedeberg's Archives of Pharmacology·S J Publicover
Jun 6, 1989·Biochimica Et Biophysica Acta·D Di JulioF F Vincenzi
Jan 1, 1982·Pharmacology & Therapeutics·J S Wiley, K E McCulloch
Jan 1, 1991·Pharmacology & Therapeutics·L MissiaenR Casteels
Jan 1, 1985·Comparative Biochemistry and Physiology. A, Comparative Physiology·J W SchmidtF F Vincenzi
Nov 1, 1983·The Journal of Cell Biology·D Bar-Sagi, J Prives
Sep 1, 1985·Plant Physiology·H D Grimes, W F Boss
Oct 1, 1982·Plant Physiology·B A Moll, R L Jones
Jan 1, 1988·Chemico-biological Interactions·L WärngårdU G Ahlborg
Jun 1, 1985·Cell Calcium·T R Hinds, F F Vincenzi
May 1, 1983·Journal of Neurochemistry·R G Sorensen, H R Mahler
Jan 1, 1982·Annals of the New York Academy of Sciences·F F Vincenzi
Dec 1, 1990·Biochemical Pharmacology·B U Raess, D M Record
Jul 9, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Janet GenzW Gary Anderson
Dec 22, 1982·Biochimica Et Biophysica Acta·R J Kurtzer, M L Roberts
Oct 15, 1982·Archives of Biochemistry and Biophysics·E J Harris, J J Heffron
Apr 13, 1999·The American Journal of Physiology·J C Fleet, R J Wood
Dec 1, 1987·Advances in Dental Research·T SasakiP R Garant
Jul 20, 2017·Scientific Reports·Hagit Bar-YosefDaniel Kornitzer
Aug 1, 1981·Cell Calcium·F F Vincenzi

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