Plasma membrane calcium ATPase isoform 3 expression in single cells isolated from rat liver.

Molecular and Cellular Biochemistry
Blanca Delgado-CoelloJ Mas-Oliva

Abstract

The plasma membrane Ca(2+)-ATPase (PMCA) located in the hepatocyte is a controversial molecule in itself since it displays different features to those regarded as canonical for P-type Ca(2+)-ATPases, and from which transcript expression as well as catalytic activity continues to be under active investigation. Our aim in this study was to explore at a first glance, pmca isoform distribution using isolated parenchymal and non-parenchymal cells from rat liver tissue. Expression of pmca transcripts was analyzed in fresh or cell-enriched culture preparations, confirming pmca1 and pmca4 as the housekeeping isoforms in all cell types studied (hepatocytes, Kupffer cells, and stellate cells). However, for the first time we show expression of pmca3 transcripts edited at two different sites in both hepatocytes and non-parenchymal cells. Interestingly, employing non-parenchymal cells we demonstrate the specific expression of pmca3e transcripts previously considered nearly exclusive of excitable tissues. Real-time PCR quantification shows a significant decrease of pmca3 transcripts in cultured Kupffer and hepatic stellate cells in comparison with fresh cells. The presence of pmca2 along with pmca3 in all liver cell types studied suggests th...Continue Reading

References

Jul 15, 1992·The Biochemical Journal·N KawadaK Decker
Jan 1, 1990·Journal of Hepatology·P Bioulac-SageC Balabaud
Jan 1, 1988·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·G BallardiniE Pisi
Apr 2, 1984·European Journal of Biochemistry·M BirmelinK Decker
Sep 1, 1995·Stroke; a Journal of Cerebral Circulation·X WangG Z Feuerstein
Oct 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M SakamotoK Tanikawa
Oct 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H Oide, R G Thurman
Oct 1, 1994·The Biochemical Journal·A HowardJ R Walters
Aug 14, 1996·Biochimica Et Biophysica Acta·B J BrownE E Strehler
Jul 18, 1997·The Journal of Biological Chemistry·N L ElwessJ T Penniston
Apr 21, 1999·Biochimica Et Biophysica Acta·H OideN Sato
Sep 2, 2000·The Journal of Biological Chemistry·T MiyaharaH Tsukamoto
Oct 12, 2000·Biochemical and Biophysical Research Communications·T M MamicG R Monteith
Jan 12, 2001·Physiological Reviews·E E Strehler, D A Zacharias
Dec 7, 2002·Seminars in Cell & Developmental Biology·Raza MalikHumphrey Hodgson
Apr 30, 2003·The Journal of Biological Chemistry·Marisa BriniErnesto Carafoli
Jun 17, 2003·Cell Structure and Function·Mitsuru SatoHaruki Senoo
Jul 5, 2003·Molecular and Cellular Biochemistry·Blanca Delgado-CoelloJaime Mas-Oliva
Sep 19, 2003·The Journal of Surgical Research·Toru MurataMasayuki Imamura
Oct 2, 2004·Nature Reviews. Molecular Cell Biology·Rebecca Taub
May 14, 2005·Journal of Virology·Masanori IsogawaFrancis V Chisari
Feb 16, 2006·Molecular and Cellular Biochemistry·Blanca Delgado-CoelloJaime Mas-Oliva
Feb 17, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Eric Santoni-RugiuHanne Cathrine Bisgaard
Apr 21, 2007·Annals of the New York Academy of Sciences·Emanuel E StrehlerAgnes Enyedi
Oct 12, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jun WuJoerg F Schlaak
Feb 23, 2008·Biochimica Et Biophysica Acta·Gregory J BarrittGrigori Y Rychkov
Dec 17, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kinji AsahinaHidekazu Tsukamoto

❮ Previous
Next ❯

Citations

Apr 4, 2015·Organogenesis·Blanca Delgado-Coello, Jaime Mas-Oliva
Jan 7, 2015·The Journal of Biological Chemistry·Genaro Vázquez-VictorioMarina Macías-Silva
Jul 6, 2014·Frontiers in Endocrinology·Marcia HiriartDeyanira Fuentes-Silva
Jan 6, 2021·The FEBS Journal·Luis Francisco CallejaJosé Salud Rodríguez-Zavala
Nov 6, 2021·Frontiers in Oncology·Marco A Briones-OrtaJaime Mas-Oliva

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.