Plasma membrane delivery of the gastric H,K-ATPase: the role of beta-subunit glycosylation

American Journal of Physiology. Cell Physiology
Olga VaginG Sachs

Abstract

The factors determining trafficking of the gastric H,K-ATPase to the apical membrane remain elusive. To identify such determinants in the gastric H,K-ATPase, fusion proteins of yellow fluorescent protein (YFP) and the gastric H,K-ATPase beta-subunit (YFP-beta) and cyan fluorescent protein (CFP) and the gastric H,K-ATPase alpha-subunit (CFP-alpha) were expressed in HEK-293 cells. Then plasma membrane delivery of wild-type CFP-alpha, wild-type YFP-beta, and YFP-beta mutants lacking one or two of the seven beta-subunit glycosylation sites was determined using confocal microscopy and surface biotinylation. Expression of the wild-type YFP-beta resulted in the plasma membrane localization of the protein, whereas the expressed CFP-alpha was retained intracellularly. When coexpressed, both CFP-alpha and YFP-beta were delivered to the plasma membrane. Removing each of the seven glycosylation sites, except the second one, from the extracellular loop of YFP-beta prevented plasma membrane delivery of the protein. Only the mutant lacking the second glycosylation site (Asn103Gln) was localized both intracellularly and on the plasma membrane. A double mutant lacking the first (Asn99Gln) and the second (Asn103Gln) glycosylation sites displayed...Continue Reading

References

May 7, 1992·Biochimica Et Biophysica Acta·K BambergG Sachs
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M A ReubenG Sachs
Jan 1, 1988·Methods in Enzymology·E C RabonG Sachs
Apr 1, 1987·The American Journal of Physiology·T Urushidani, J G Forte
Oct 1, 1983·The American Journal of Physiology·A SmolkaG Sachs
Nov 2, 1995·Nature·P ScheiffeleK Simons
Feb 1, 1995·The American Journal of Physiology·C J GottardiM J Caplan
Aug 1, 1994·Current Opinion in Cell Biology·K Matter, I Mellman
Apr 1, 1993·The Journal of Cell Biology·C J Gottardi, M J Caplan
Apr 1, 1996·The Journal of Cell Biology·T YoshimoriK Simons
Aug 1, 1997·Current Opinion in Cell Biology·L M Traub, S Kornfeld
Dec 4, 1998·Seminars in Cell & Developmental Biology·E Ikonen, K Simons
Jan 29, 1999·Physiological Reviews·C YeamanW J Nelson
Feb 15, 2001·Nature Cell Biology·P KellerK Simons
Mar 23, 2002·The Journal of Biological Chemistry·Tohru KimuraShinji Asano
Jul 26, 2002·Nature·Yukiko YoshidaTadashi Tai

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Citations

Jun 10, 2008·Pflügers Archiv : European journal of physiology·Jai Moo ShinGeorge Sachs
Nov 14, 2008·Current Gastroenterology Reports·Jai Moo Shin, George Sachs
Sep 8, 2010·Current Gastroenterology Reports·Sascha Kopic, John P Geibel
Jul 28, 2001·Development, Growth & Differentiation·K AngioiA Duprez
Aug 19, 2007·International Immunology·Desmond K Y AngIan R van Driel
Mar 9, 2007·American Journal of Physiology. Renal Physiology·Melissa D LaugheryJack H Kaplan
Nov 2, 2019·Physiological Reviews·Amy C EngevikJames R Goldenring
Jan 11, 2013·Physiological Reviews·Sascha Kopic, John P Geibel

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