PMID: 9547365May 9, 1998Paper

Plasma membrane depolarization and disturbed Na+ homeostasis induced by the protonophore carbonyl cyanide-p-trifluoromethoxyphenyl-hydrazon in isolated nerve terminals

Molecular Pharmacology
L TretterV Adam-Vizi

Abstract

The effect of the protonophore carbonyl cyanide-p-trifluoromethoxyphenyl-hydrazon (FCCP) was studied on the intracellular [Na+], pH, and plasma membrane potential in isolated nerve terminals. FCCP induced a rise of [Na+]i at, and even below, the concentrations (0.025-1 microM) in which it is usually used in intact cells to eliminate Ca2+ uptake by mitochondria. The FCCP-induced increase of [Na+]i correlates with a fall in both the ATP level and the ATP/ADP ratio. In addition, a sudden rise of the intracellular proton concentration ([H+]i) from 83 +/- 0.4 to 124 +/- 0.7 nM was observed on the addition of FCCP (1 microM). Parallel with the rise in [H+]i, an abrupt depolarization was detected, followed by a slower decrease in the plasma membrane potential. Both the extent of the pHi change and the fast depolarization of the plasma membrane were proportional to the proton electrochemical gradient across the plasma membrane; when this gradient was increased, greater depolarization was detected. The slower decrease of the membrane potential after the fast initial depolarization was abolished when the medium contained no Na+. It is concluded that FCCP (1) gives rise to a depolarization by setting the plasma membrane potential close to...Continue Reading

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Citations

Jan 25, 2003·European Journal of Biochemistry·Chatchanok LoetchutinatArlette Garnier-Suillerot
Feb 1, 2007·Journal of Neurochemistry·Laszlo TretterVera Adam-Vizi
Apr 22, 2011·British Journal of Pharmacology·Daniel José BarbosaFélix Carvalho
Apr 6, 2019·Nature Communications·Pasquale D'AcunzoFrancesco Cecconi
Sep 24, 2020·Biomolecules·Svyatoslav S SokolovFedor F Severin
Jan 5, 2000·Physiological Reviews·D G Nicholls, S L Budd
Jul 27, 2012·Journal of Neurophysiology·Michael G WhiteJohn N Barrett
May 21, 1999·The European Journal of Neuroscience·C BoyerJ Lehouelleur
Jul 11, 2014·PloS One·Divya PadmarajWanda Zagozdzon-Wosik
Jan 9, 2015·Journal of Neurophysiology·Yi-Chi WangRong-Chi Huang
Jun 21, 2014·Naunyn-Schmiedeberg's Archives of Pharmacology·Hartmut GlaeserKathrin Mandery
Sep 25, 2020·Bioelectrochemistry·Alexander M FirsovYuri N Antonenko
Aug 25, 2020·Toxicology and Applied Pharmacology·Suraj SangroulaJulie A Gosse
Nov 12, 2003·Biochemical Pharmacology·Bettina FroestlWalter E Müller
Jan 31, 2006·Free Radical Biology & Medicine·Hildegard LaggnerHans Goldenberg
Dec 26, 2006·Progress in Neurobiology·Diane T W Chang, Ian J Reynolds
May 15, 2007·Brain Research Bulletin·Tatyana V WaseemSergei V Fedorovich

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