PMID: 58927May 1, 1976

Plasma membrane localization of alkaline phosphatase in HeLa cells

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
C W LinR M Singer


The localization of alkaline phosphatase in HeLa cells was examined by electron microscopic histochemistry and subcellular fractionation techniques. Two monophenotypic sublines of HeLa cells which respectively produced Regan and non-Regan isoenzymes of alkaline phosphatase were used for this study. The electron microscopic histochemical results showed that in both sublines the major location of alkaline phosphatase is in the plasma membrane. The enzyme reaction was occasionally observed in some of the dense body lysosomes. This result was supported by data obtained from a subcellular fractionation study which showed that the microsomal fraction rich in plasma membrane fragments had the highest activity of alkaline phosphatase. The distribution of this enzyme among the subcellular fractions closely paralleled that of the 5'-nucleotidase, a plasma membrane marker enzyme. Characterization of the alkaline phosphatase present in each subcellular fraction showed identical enzyme properties, which suggests that a single isoenzyme exists among fractions obtained from each cell line. The results, therefore, confirm the reports suggesting that plasma membrane is the major site of alkaline phosphatase localization in HeLa cells. The absen...Continue Reading


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Related Concepts

Alkaline Phosphatase
Cell Fractionation
Plasma Membrane
HeLa Cells
Electron Microscopy
Staining and Labeling
Subcellular Fractions

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