Plasma membrane localization of multidrug resistance-associated protein homologs in brain capillary endothelial cells

The Journal of Pharmacology and Experimental Therapeutics
Yan ZhangDonald W Miller

Abstract

Several multidrug resistance-associated protein (MRP) homologs are expressed in brain microvessel endothelial cells forming the blood-brain barrier (BBB). The influence of these MRP transporters on BBB permeability will be dependent on their localization within the brain microvessel endothelial cells. Using two different and complementary approaches, the localization of various MPR homologs (MRP1, MRP4, and MRP5) was examined in primary cultured bovine brain microvessel endothelial cells (BBMECs). The first approach involved centrifugal separation of apical and basolateral plasma membranes of cultured BBMECs. The membrane fractions were then subjected to Western blot analysis for MRPs. The second approach used confocal laser scanning microscopy to determine membrane localization of MRPs in BBMECs. Results show a predominantly apical plasma membrane distribution for MRP1 and MRP5, and an almost equal distribution of MRP4 on the apical and basolateral plasma membrane of BBMECs. These studies provide the first demonstration of the localization of MRP1, MRP4, and MRP5 homologs in brain microvessel endothelial cells. The present studies also indicate that the localization of MRPs in the endothelial cells forming the BBB is different...Continue Reading

References

Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C Cordon-CardoJ R Bertino
Jan 1, 1987·Journal of Neuroscience Research·J B FishmanR E Fine
Jun 23, 1995·The Journal of Biological Chemistry·M M Sánchez del PinoR A Hawkins
Mar 1, 1996·Molecular Medicine Today·N J Abbott, I A Romero
Mar 17, 1998·Biochemical and Biophysical Research Communications·H Huai-YunD W Miller
Sep 18, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B GaoP J Meier
Dec 3, 1999·Biochimica Et Biophysica Acta·P BorstJ Wijnholds
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·J WijnholdsP Borst
Aug 17, 2000·Journal of the National Cancer Institute·P BorstJ Wijnholds
Dec 7, 2000·Journal of the National Cancer Institute·K LeeG D Kruh
Mar 30, 2001·The Journal of Biological Chemistry·I A SimpsonR A Hawkins
Feb 22, 2002·Journal of the American Society of Nephrology : JASN·Rémon A M H van AubelFrans G M Russel
Jan 22, 2003·Advanced Drug Delivery Reviews·Alfred H Schinkel, Johan W Jonker
Jun 24, 2003·Pharmaceutical Research·Salvatore CisterninoJean-Michel Scherrmann

❮ Previous
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Citations

Sep 4, 2009·European Journal of Clinical Pharmacology·Bradley L Urquhart, Richard B Kim
Jul 19, 2006·Pflügers Archiv : European journal of physiology·Anne T Nies, Dietrich Keppler
Dec 26, 2006·Pflügers Archiv : European journal of physiology·Eva Bakos, László Homolya
Jan 22, 2003·Advanced Drug Delivery Reviews·Haiying SunWilliam F Elmquist
Jun 17, 2011·Expert Reviews in Molecular Medicine·Sagar AgarwalWilliam F Elmquist
Mar 7, 2006·Nature Reviews. Drug Discovery·Gergely SzakácsMichael M Gottesman
Jul 19, 2005·Nature Reviews. Neuroscience·Wolfgang Löscher, Heidrun Potschka
Nov 23, 2012·Biologie aujourd'hui·Vincent BérézowskiRoméo Cecchelli
Mar 28, 2009·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Pradeep K KarlaAshim Mitra
Oct 2, 2007·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Deryk SequeiraBurhan Mahmood
Jun 11, 2005·Epilepsia·Dieter Schmidt, Wolfgang Löscher
Jan 28, 2010·Pharmacological Reviews·Curtis D Klaassen, Lauren M Aleksunes
Oct 12, 2010·Journal of Angiogenesis Research·Ariel Gilert, Marcelle Machluf
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Tetsuya Terasaki, Sumio Ohtsuki
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Hiroyuki Kusuhara, Yuichi Sugiyama
Apr 6, 2006·Pflügers Archiv : European journal of physiology·Piet BorstKoen van de Wetering
Jun 28, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Reina BendayanMoise Bendayan
Jan 30, 2014·Drug Delivery·Narayanan KasinathanJ Venkata Rao
Mar 29, 2014·Drug Metabolism Reviews·Tereza Kunická, Pavel Souček
Aug 23, 2006·Expert Opinion on Drug Metabolism & Toxicology·Jean-Michel Scherrmann
Dec 22, 2012·Expert Opinion on Drug Metabolism & Toxicology·Xiaoyan ChuRaymond Evers
Jul 3, 2009·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Anne MahringerGert Fricker
Mar 20, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Björn BauerDavid S Miller
Jul 16, 2008·European Journal of Pharmacology·Michelle L HollandJonathon C Arnold
Apr 17, 2007·Brain Research·Nicolas PerrièrePierre-Olivier Couraud
Dec 29, 2006·Journal of Clinical Pharmacology·Anne MannilaHannu Kokki
Oct 19, 2006·Annals of Neurology·Dirk M HermannClaudio L Bassetti
May 16, 2015·Cellular and Molecular Neurobiology·Guang-Yun WangHua-Ning Liao

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