Plasma metabolomics reveals lower carnitine concentrations in overweight Labrador Retriever dogs

Acta Veterinaria Scandinavica
Josefin SöderS Wernersson

Abstract

The prevalence of overweight is increasing in dogs, but the metabolic events related to this condition are still poorly understood. The purpose of the study was to investigate the postprandial response of plasma metabolites using a meal-challenge test and to identify metabolic variations related to spontaneous overweightness in privately owned dogs. Twenty-eight healthy male intact Labrador Retriever dogs were included, 12 of which were classified as lean (body condition score (BCS) 4-5 on a 9-point scale) and 16 as overweight (BCS 6-8). After an overnight fast (14-17 h), blood samples were collected and dogs were thereafter fed a high-fat meal. Postprandial blood samples were collected hourly four times. Plasma metabolites were identified by nuclear magnetic resonance. Postprandial metabolomes differed from the fasting metabolome in multivariate discriminant analysis (PLS-DA: Q2Y = 0.31-0.63, cross-validated ANOVA: P ≤ 0.00014) Eleven metabolites, all amino acids, contributed to the separations. Carnitine was identified as a metabolite related to overweight (stepwise logistic regression analysis P ≤ 0.03) and overweight dogs had overall lower carnitine response (mixed model repeated measures analysis P = 0.005) than lean dogs....Continue Reading

References

Jul 1, 1990·JPEN. Journal of Parenteral and Enteral Nutrition·R J Smith
Aug 1, 1989·The American Journal of Clinical Nutrition·K A LombardC J Rebouche
Apr 1, 1987·The American Journal of Clinical Nutrition·G Cederblad
Oct 1, 1983·Physiological Reviews·J Bremer
May 1, 1980·The American Journal of Physiology·C L Hoppel, S M Genuth
Nov 5, 1998·Sports Medicine·N P WalshM Gleeson
May 10, 2001·The Journal of Clinical Endocrinology and Metabolism·C WeyerP A Tataranni
May 7, 2002·Journal of the American Veterinary Medical Association·Richard D KealyHoward D Stowe
Jul 18, 2002·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·O Bosello, M Zamboni
Apr 3, 2004·Journal of Animal Physiology and Animal Nutrition·M DiezV Biourge
Mar 7, 2006·JPEN. Journal of Parenteral and Enteral Nutrition·Soh IwashitaPaul J Flakoll
Jun 15, 2006·The Journal of Nutrition·Alexander J German
Nov 7, 2006·The Veterinary Clinics of North America. Small Animal Practice·Sherry Lynn Sanderson
Jun 16, 2007·Epidemiologic Reviews·Benjamin Caballero
Dec 8, 2007·The British Journal of Nutrition·Dennis F LawlerRichard D Kealy
Jun 24, 2009·Magnetic Resonance in Chemistry : MRC·Patrick A Hays, Robert A Thompson
Jun 26, 2009·The Journal of Biological Chemistry·Robert C NolandDeborah M Muoio
Jan 1, 2009·Metabolomics : Official Journal of the Metabolomic Society·Angela M ZivkovicJ Bruce German
Nov 15, 2011·The Veterinary Journal·A J GermanE M Scott
Mar 27, 2012·Metabolomics : Official Journal of the Metabolomic Society·Linette PellisSuzan Wopereis
Aug 30, 2012·BMC Veterinary Research·Asta TvarijonaviciuteAlexander J German
Dec 22, 2012·Diabetes·Marieke G SchoonemanMaarten R Soeters
Jan 3, 2013·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·A ZhangX Wang
Nov 13, 2013·Journal of the American Animal Hospital Association·Dawn BrooksAngela Witzel
Dec 20, 2014·The Veterinary Record·Peter SandøeCharlotte Reinhard Bjørnvad
Jan 30, 2016·Journal of Veterinary Internal Medicine·J SöderK Höglund
Sep 21, 2016·BMC Veterinary Research·Asta TvarijonaviciuteAlexander J German
Jan 7, 2018·The Veterinary Record·Alexander J GermanCaroline Burke

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Citations

Dec 1, 2019·GeroScience·Jessica M HoffmanSteven N Austad
Sep 22, 2020·PloS One·Mariana Yukari Hayasaki PorsaniMarcio Antonio Brunetto
Feb 18, 2021·Metabolomics : Official Journal of the Metabolomic Society·Thiago H A VendraminiMarcio A Brunetto
Mar 6, 2021·Scientific Reports·Sonu YadavJ William O Ballard

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Methods Mentioned

BETA
NMR
blood collection
PCA
X-ray

Software Mentioned

AQuA
TopSpin
ChenomX
SIMCA
SAS

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