Plasma miR-185 is decreased in patients with esophageal squamous cell carcinoma and might suppress tumor migration and invasion by targeting RAGE

American Journal of Physiology. Gastrointestinal and Liver Physiology
Rongrong JingMing Cui

Abstract

The receptor for advanced-glycation end products (RAGE) is upregulated in various cancers and has been associated with tumor progression, but little is known about its expression and regulation by microRNAs (miRNAs) in esophageal squamous cell carcinoma (ESCC). Here, we describe miR-185, which represses RAGE expression, and investigate the biological role of miR-185 in ESCC. In this study, we found that the high level of RAGE expression in 29 pairs of paraffin-embedded ESCC tissues was correlated positively with the depth of invasion by immunohistochemistry, suggesting that RAGE was involved in ESCC. We used bioinformatics searches and luciferase reporter assays to investigate the prediction that RAGE was regulated directly by miR-185. Besides, overexpression of miR-185 in ESCC cells was accompanied by 27% (TE-11) and 49% (Eca-109) reduced RAGE expression. The effect was further confirmed in RAGE protein by immunofluorescence in both cell lines. The effects were reversed following cotransfection with miR-185 and high-level expression of the RAGE vector. Furthermore, the biological role of miR-185 in ESCC cell lines was investigated using assays of cell viability, Ki-67 staining, and cell migration and invasion, as well as in a ...Continue Reading

References

Apr 21, 2007·The EMBO Journal·Russell L DuringFrederick S Southwick
Mar 15, 2008·Cancer Research·Minalini LakshmanRaymond C Bergan
Oct 24, 2009·Nucleic Acids Research·Tian Sheng ChenSai Kiang Lim
Jan 21, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rie HamanoYuichiro Doki
Dec 31, 2011·Biochemical and Biophysical Research Communications·Indira ElangovanMunirathinam Gnanasekar
Jul 28, 2012·Current Cancer Drug Targets·Hailin TangMinghua Wu
May 25, 2013·Journal of the National Cancer Institute·Yuji ToiyamaAjay Goel
Jan 21, 2014·European Journal of Histochemistry : EJH·X C XuY H Wang
Mar 15, 2014·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Masahiro TsujiuraEigo Otsuji
May 9, 2014·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·F XuB Qian
May 14, 2014·Journal of Molecular and Cellular Cardiology·Appledene OsbourneGene H Kim
May 14, 2014·Oncology Reports·Jufeng ZhangYanxin Lu
Oct 19, 2014·Cancer Research·Xuebo ChenBehnam Badie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.