PMID: 6171609Oct 1, 1981Paper

Plasma myelin basic protein assay using Gilford enzyme immunoassay cuvettes

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie
N P Groome

Abstract

The assay of myelin basic protein in body fluids has potential clinical importance as a routine indicator of demyelination. Preliminary details of a competitive enzyme immunoassay for this protein have previously been published by the author (Groome, N. P. (1980) J. Neurochem. 35, 1409-1417). The present paper now describes the adaptation of this assay for use on human plasma and various aspects of routine data processing. A commercially available cuvette system was found to have advantages over microtitre plates but required a permuted arrangement of sample replicates for consistent results. For dose interpolation, the standard curve could be fitted to a three parameter non-linear equation by regression analysis or linearised by the logit/log transformation.

References

Nov 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·A H Schuurs, B K Van Weemen
Jan 16, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·J W PalfreymanJ G Ratcliffe
Jan 1, 1980·Journal of Immunological Methods·R M Hendry, J E Herrmann
Jan 1, 1980·Journal of Immunological Methods·O P Lehtonen, M K Viljanen

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Citations

Jan 1, 1983·Neurochemistry International·N P GroomeJ Appleby

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