Plasma nitrite rather than nitrate reflects regional endothelial nitric oxide synthase activity but lacks intrinsic vasodilator action

Proceedings of the National Academy of Sciences of the United States of America
T LauerM Kelm

Abstract

The plasma level of NO(x), i.e., the sum of NO(2)- and NO(3)-, is frequently used to assess NO bioavailability in vivo. However, little is known about the kinetics of NO conversion to these metabolites under physiological conditions. Moreover, plasma nitrite recently has been proposed to represent a delivery source for intravascular NO. We therefore sought to investigate in humans whether changes in NO(x) concentration are a reliable marker for endothelial NO production and whether physiological concentrations of nitrite are vasoactive. NO(2)- and NO(3)- concentrations were measured in blood sampled from the antecubital vein and brachial artery of 24 healthy volunteers. No significant arterial-venous gradient was observed for either NO(2)- or NO(3)-. Endothelial NO synthase (eNOS) stimulation with acetylcholine (1-10 microg/min) dose-dependently augmented venous NO(2)- levels by maximally 71%. This effect was paralleled by an almost 4-fold increase in forearm blood flow (FBF), whereas an equieffective dose of papaverine produced no change in venous NO(2)-. Intraarterial infusion of NO(2)- had no effect on FBF. NOS inhibition (N(G)-monomethyl-l-arginine; 4-12 micromol/min) dose-dependently reduced basal NO(2)- and FBF and blunte...Continue Reading

References

Nov 1, 1990·British Journal of Pharmacology·D D ReesS Moncada
Jan 1, 1990·Annual Review of Pharmacology and Toxicology·L J Ignarro
Oct 23, 1986·The New England Journal of Medicine·P L LudmerP Ganz
Aug 25, 1988·The New England Journal of Medicine·T F LüscherE Weber
Jul 15, 1988·Biochemical and Biophysical Research Communications·M KelmJ Schrader
Jul 1, 1981·Journal of Inorganic Biochemistry·M P Doyle, J W Hoekstra
Jan 1, 1993·Journal of Cardiovascular Pharmacology·G M Rubanyi
Oct 1, 1994·Microscopy Research and Technique·K AddicksM Feelisch
Jun 1, 1995·Annals of Medicine·R Busse, I Fleming
Jul 15, 1995·Lancet·P VallanceT Malinski
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L J IgnarroR E Byrns
Apr 17, 1995·Biochemical and Biophysical Research Communications·P RhodesP ] Rhodes PM [corrected to Rhodes
Apr 18, 1994·Annals of the New York Academy of Sciences·A WennmalmF Waagstein
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·J R Lancaster
Jul 12, 1993·FEBS Letters·P C FordD M Stanbury
Jun 4, 1996·Biochemistry·R F EichJ S Olson
Jan 1, 1996·Nutrition·H M Delany
Nov 1, 1996·The American Journal of Physiology·J S Beckman, W H Koppenol
Feb 1, 1996·Methods : a Companion to Methods in Enzymology·R S Athwal, G P Kaur
Jul 21, 1998·The Journal of Biological Chemistry·X LiuJ R Lancaster
Nov 14, 1998·Biochimica Et Biophysica Acta·A R ButlerP G Wright
Jan 14, 1999·The New England Journal of Medicine·R Ross
May 13, 1999·Biochimica Et Biophysica Acta·M Kelm
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·J C LiaoL Kuo
Aug 12, 1999·Nitric Oxide : Biology and Chemistry·K SchulzM Kelm
Oct 12, 1999·Clinical Physiology·E CicinelliN Falco
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·M T GladwinR O Cannon
Nov 18, 2000·American Journal of Physiology. Cell Physiology·J M MayC E Cobb
Nov 25, 2000·The Journal of Biological Chemistry·H M Abu-Soud, S L Hazen
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·A NedospasovE Nudler
Dec 29, 2000·Ultraschall in der Medizin : Organ der Deutschen Gesellschaft für Ultraschall in der Medizin, [der] Österreichischen Gesellschaft für Ultraschall in der Medizin, [der] Schweizerischen Gesellschaft für Ultraschall in Medizin und Biologie·M PreikM Kelm
Mar 30, 1979·Science·D S Woodruff

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Citations

May 4, 2006·Archives of Toxicology·Fernando BarbosaJose E Tanus-Santos
Aug 10, 2010·Naunyn-Schmiedeberg's Archives of Pharmacology·Marcelo F MontenegroJose E Tanus-Santos
Jan 21, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·Marcelo F MontenegroJose E Tanus-Santos
May 21, 2013·Naunyn-Schmiedeberg's Archives of Pharmacology·Jaqueline J MunizJose E Tanus-Santos
Sep 1, 2004·Histochemistry and Cell Biology·Barbara VoetschJoseph Loscalzo
Nov 7, 2007·European Journal of Applied Physiology·Urs A LeuenbergerDavid A MacLean
Feb 21, 2013·European Journal of Applied Physiology·Anni VanhataloAndrew M Jones
Sep 18, 2007·Pflügers Archiv : European journal of physiology·D TrigueroA García-Pascual
Jan 18, 2003·Current Hypertension Reports·Malte Kelm
Sep 4, 2009·Archives of Pharmacal Research·David J Lefer
Jun 7, 2013·Aging Clinical and Experimental Research·Hugo A P SantanaHerbert G Simões
May 4, 2004·Blood Cells, Molecules & Diseases·André DejamMark T Gladwin
Jun 15, 2007·Cardiovascular Research·Cameron DezfulianMark T Gladwin
Mar 3, 2004·Free Radical Biology & Medicine·Mark T GladwinRakesh P Patel
Mar 31, 2004·Nitric Oxide : Biology and Chemistry·Anthony R Butler, John H Ridd
Nov 26, 2002·Free Radical Biology & Medicine·Tienush RassafMartin Feelisch
Oct 30, 2003·Free Radical Biology & Medicine·Petra KleinbongardMalte Kelm
May 3, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Frank B Jensen
Nov 6, 2007·Nature Chemical Biology·Swati BasuDaniel B Kim-Shapiro
Sep 14, 2004·Nature Medicine·Christian J HunterMark T Gladwin
Jan 3, 2008·Nature Reviews. Drug Discovery·Jon O LundbergMark T Gladwin
Jun 16, 2004·Nature Reviews. Microbiology·Jon O LundbergNigel Benjamin
Feb 14, 2002·British Journal of Haematology·Mark T GladwinGriffin P Rodgers
Jun 26, 2003·Clinical and Experimental Pharmacology & Physiology·Aafje SierksmaHenk F J Hendriks
Jul 25, 2003·The New England Journal of Medicine·Jonathan S Stamler
Mar 15, 2013·The New England Journal of Medicine·Bill Giannakopoulos, Steven A Krilis
Apr 12, 2012·The Journal of Biological Chemistry·Jesús TejeroMark T Gladwin
Jun 1, 2005·Nutrition Research Reviews·Wendy L HallChristine M Williams
Jul 29, 2010·The Journal of Infectious Diseases·Jacqueline J JankaDonald J Krogstad
Aug 17, 2006·Antioxidants & Redox Signaling·Argirios E TsantesNikolaos M Sitaras
Jan 15, 2013·Antioxidants & Redox Signaling·Michele UmbrelloMervyn Singer
Mar 3, 2009·Cardiovascular Research·John W Calvert, David J Lefer
Sep 21, 2010·Cardiovascular Research·Christopher B PattilloChristopher G Kevil
Oct 5, 2010·Cardiovascular Research·Julian O M OrmerodMichael P Frenneaux
Oct 5, 2010·Cardiovascular Research·Mark GilchristNigel Benjamin
Oct 19, 2010·Cardiovascular Research·Rakesh P PatelDaniel B Kim-Shapiro
May 15, 2010·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Graciela Aurora RuizMartin Nogues
Jan 14, 2011·Biology of Reproduction·Hong-hai ZhangDong-bao Chen
Jun 24, 2006·Journal of Cardiovascular Pharmacology·Jan BalzerMalte Kelm
Aug 13, 2005·Critical Care Medicine·Sruti Shiva, Mark T Gladwin
Jun 1, 2007·Journal of Addiction Medicine·Marc J KaufmanDomenic A Ciraulo
Jun 12, 2010·Cardiology in Review·Vaughn E NossamanPhilip J Kadowitz

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