Plasma phospholipids implicated in the matrix effect observed in liquid chromatography/tandem mass spectrometry bioanalysis: evaluation of the use of colloidal silica in combination with divalent or trivalent cations for the selective removal of phospholipids from plasma

Rapid Communications in Mass Spectrometry : RCM
Steven T WuMohammed Jemal

Abstract

The feasibility of the use of colloidal silica in combination with a number of divalent or trivalent cations for the removal of plasma phospholipids was evaluated by sequentially adding the two reagents (i.e., colloidal silica and a cation) directly to blank plasma samples or plasma samples spiked with analytes. Three representative plasma phospholipids were monitored to determine the efficiency of the phospholipids removal under different reagent combinations. The recovery of each spiked analyte was also monitored under each condition in order to determine if any of the analyte was removed along with the phospholipids. By optimizing the amounts of the reagents used and the sequence of the addition of the reagents, quantitative and reproducible removal of the phospholipids was achieved. Using the finally selected lanthanum cation, the removal of phospholipids was achieved with minimal concomitant loss of the ten investigated analytes which were carefully selected to incorporate functional groups that could potentially interact with the added reagents and hence could be removed along with the phospholipids.

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Citations

Nov 19, 2010·Bioanalysis·Cynthia CôtéFabio Garofolo
Apr 1, 2009·Bioanalysis·Jean-Nicholas MessFabio Garofolo
Jul 16, 2011·Bioanalysis·Zhengqi YeChristopher L Brummel
Nov 17, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chang-Ik ChoiSeok-Yong Lee
Jan 13, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chang-Ik ChoiSeok-Yong Lee
Jul 8, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei ZengEric J Woolf
Jun 26, 2009·Journal of Pharmaceutical and Biomedical Analysis·Vincenzo PucciEdith Monteagudo
Apr 19, 2011·Mass Spectrometry Reviews·Helga TrufelliAchille Cappiello
Feb 16, 2010·Journal of Separation Science·Wenying JianNaidong Weng
Jan 17, 2016·Biomedical Chromatography : BMC·Jennifer Carmical, Stacy Brown
May 14, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jixia WangXinmiao Liang
Dec 12, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chang-Ik ChoiSeok-Yong Lee
Jan 24, 2020·The Analyst·Marco Beccaria, Deirdre Cabooter

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