PMID: 6406546May 1, 1983Paper

Plasma postheparin diamine oxidase. Sensitive provocative test for quantitating length of acute intestinal mucosal injury in the rat

The Journal of Clinical Investigation
G D LukS B Baylin

Abstract

Diamine oxidase (DAO; EC 1.4.3.6) is an enzyme found in high activity in the mature cells of the upper villus of rat small intestinal mucosa and in very much lower activity in all other tissues in the nonpregnant rat. This study was designed to determine whether a provocative test for increasing the level of plasma DAO activity by heparin administration could be used to monitor the extent and severity of acute, severe, small intestinal mucosal injury. In adult rats, small intestinal loops of varying lengths were perfused with 2,100 mosM sodium sulfate solution for 60 min to produce selective damage to villus epithelium. Plasma postheparin DAO (PHD) activity (180 min after 400 U/kg i.p. heparin) was measured 7 h after initiation of perfusion. With increasing length of intestinal mucosal injury, there was a progressive decrease in both basal and plasma PHD activity. The decrease in plasma PHD activity closely reflected the length of intestinal mucosa injured (n = 128, r = 0.86, P less than 0.001), and it was much more sensitive (threshold limit of detection = 13% of total length, range = 67 U/ml for 100% length of injury) than unstimulated basal levels of plasma DAO (threshold = 40%, range = 2.1 U/ml). Our previous data have sugg...Continue Reading

References

Jun 1, 1977·Gastroenterology·P F WhittingtonT M Bayless
Jan 1, 1979·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·S L Taylor, E R Lieber
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·O DahlbäckN Tryding
Jul 1, 1969·Biochemical Pharmacology·Y KobayashiD V Maudsley
May 1, 1980·The New England Journal of Medicine·D H PresentB S Pasternack
Jul 1, 1980·The Journal of Clinical Investigation·G D LukS B Baylin
Jan 1, 1964·Analytical Biochemistry·A DAHLQVIST

❮ Previous
Next ❯

Citations

Nov 1, 1991·Digestive Diseases and Sciences·J S ThompsonW P Vaughan
Mar 1, 1987·Digestive Diseases and Sciences·L D'AgostinoG Mazzacca
Nov 1, 1984·Digestive Diseases and Sciences·L D'AgostinoG Mazzacca
Aug 1, 1988·Digestive Diseases and Sciences·G R CorazzaG Gasbarrini
Nov 1, 1994·Agents and Actions·A Sessa, A Perin
Jan 1, 1989·Biochemical Pharmacology·L D'AgostinoG Mazzacca
Jan 1, 1994·Transplant International : Official Journal of the European Society for Organ Transplantation·R W de BruinR L Marquet
Feb 6, 2013·World Journal of Gastroenterology : WJG·Wei LiuZhi Liu
Aug 1, 1989·The Journal of Surgical Research·N HosodaM Yamamoto
May 15, 2015·Journal of Gastroenterology and Hepatology·Jinsei MiyoshiTetsuji Takayama
Mar 1, 1991·The Journal of Surgical Research·S G RoseE M Quigley
Jul 13, 2012·Drug Metabolism and Pharmacokinetics·Keiichiro HigashiKen-ichi Miyamoto
Apr 8, 2014·The Journal of Medical Investigation : JMI·Hirokazu TakechiAkira Tangoku
Feb 5, 2014·JPEN. Journal of Parenteral and Enteral Nutrition·Rie TsutsumiYasuo M Tsutsumi
Mar 1, 1992·JPEN. Journal of Parenteral and Enteral Nutrition·H TamadaA Okada
Jul 1, 1985·Physical Review A: General Physics·T S LukC K Rhodes
Feb 4, 2014·The International Journal of Eating Disorders·Yoshiyuki TakimotoAkira Akabayashi
Jan 1, 1990·Annals of the New York Academy of Sciences·H WadaY Inada
May 17, 2007·Shock·Li-Tian ZhangZhi-Yong Sheng
Mar 10, 2016·Molecular Medicine Reports·Sisi FengBaiyun Zhong

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.