Plasma protein adsorption patterns on emulsions for parenteral administration: establishment of a protocol for two-dimensional polyacrylamide electrophoresis

Electrophoresis
S Harnisch, R H Müller

Abstract

The two-dimensional polyacrylamide electrophoresis (2-D PAGE) of the plasma protein adsorption pattern previously established for polymeric nanoparticles was modified and transferred to oil in water emulsions for intravenous administration. The emulsions were incubated with citrated plasma, and separation from excess plasma was performed by centrifugation under optimized conditions: 15000 g and three washing steps with 0.05 M phosphate buffer, pH 7.4. With this sample preparation, coalescence of droplets could be avoided and an unchanged surface area maintained, in addition the phosphate buffer minimized artificial IgG adsorption. Critical factors affecting sensitivity were contamination of the sample by oil residues and the use of thiourea in the immobilized pH gradients. Changes in the protein adsorption pattern caused by altered surface properties of the emulsion (i.e. adsorbed Poloxamer 407) were detectable when applying the optimized protocol. Knowledge of the protein adsorption patterns and their correlation to in vivo behavior opens the perspective for the development of intravenous emulsions for controlled drug delivery.

References

Nov 1, 1991·Electrophoresis·N L Anderson, N G Anderson
Mar 1, 1989·Journal of Biomedical Materials Research·J H LeeJ D Andrade
Sep 1, 1988·Analytical Biochemistry·D F HochstrasserC R Merril
Nov 1, 1993·Electrophoresis·O GolazC Walzer
May 1, 1996·Electrophoresis·T Rabilloud
Oct 1, 1992·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·H CarrstensenB W Müller

❮ Previous
Next ❯

Citations

Mar 4, 2000·International Journal of Pharmaceutics·K SeehofR H Müller
Mar 4, 2000·International Journal of Pharmaceutics·A GessnerR H Müller
Aug 23, 2002·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Andrea GessnerRainer Müller
Dec 30, 1999·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·S Harnisch, R H Müller
Mar 8, 2000·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·K BuszelloB W Müller
May 15, 2003·Journal of Biomedical Materials Research. Part a·Andrea GessnerRainer H Müller
Nov 26, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Ranjita ShegokarRainer H Müller
Oct 1, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Medha D Joshi, Rainer H Müller
Dec 8, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·B PetriS Gelperina
Nov 30, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Shunmugaperumal TamilvananSimon Benita
Jan 12, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Andreas LemkeOliver Kayser
May 4, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Carsten OlbrichRainer H Müller
Aug 16, 2005·International Journal of Pharmaceutics·T M Göppert, R H Müller
Feb 22, 2012·International Journal of Pharmaceutics·M JanschR H Müller
Jun 21, 2015·Canadian Association of Radiologists Journal = Journal L'Association Canadienne Des Radiologistes·Kieran MurphyChett Boxley
Nov 5, 2014·Interventional Neuroradiology : Journal of Peritherapeutic Neuroradiology, Surgical Procedures and Related Neurosciences·Johannes Gossner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.