Plasma protein biomarkers of hepatocellular carcinoma in HCV-infected alcoholic patients with cirrhosis

PloS One
Gustavo FerrínManuel De la Mata

Abstract

Hepatocellular carcinoma (HCC) is one of the most common and lethal cancers in the world, with limited options for treatment unless timely diagnosed. Chronic hepatitis C virus (HCV) infection and persistent heavy alcohol consumption are independent risk factors for HCC development, which may induce a specific protein expression pattern different from those caused separately. The aim of the study was to identify protein biomarkers for the detection of HCC in HCV-infected alcoholic patients with cirrhosis in order to improve survival. We compared protein expression profiles of plasma samples from 52 HCV-infected alcoholic patients with and without HCC, using 2-D DIGE coupled with MALDI-TOF/TOF mass spectrometry. The 2-D DIGE results were analyzed statistically using Decyder software, and verified by western-blot and ELISA. In plasma samples from HCV-infected alcoholic patients, we found significantly differential expression profiles of carboxypeptidase-N, ceruloplasmin (CP), complement component 4a (C4a), fibrinogen-alpha (FGA), immunoglobulin mu chain C region, serum albumin, and serum paraoxonase/arylesterase 1 (PON1). Deregulation of plasma/serum levels of the identified proteins was associated to HCV, ethanol consumption, and...Continue Reading

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Citations

Mar 30, 2017·Expert Review of Gastroenterology & Hepatology·Eva Juárez-HernándezVarenka Barbero Becerra
May 6, 2020·The Journal of Clinical Investigation·Jennifer LaskowskiJoshua M Thurman
Dec 13, 2018·Virusdisease·Kruti DalalAruna Shankarkumar
Aug 1, 2019·World Journal of Clinical Cases·Lavinia Alice Balaceanu
Jul 8, 2017·World Journal of Clinical Cases·Puneet GandhiSandeep Sorte
Sep 14, 2021·Immunity, Inflammation and Disease·Mingde LiXingxing Huo

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Methods Mentioned

BETA
electrophoresis
ELISA
biopsy
imaging techniques

Software Mentioned

Decyder
MASCOT

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