PMID: 12771370May 29, 2003Paper

Plasma protein synthesis measurements using a proteomics strategy

The Journal of Nutrition
H M H van Eijk, N E P Deutz

Abstract

The analysis of the synthesis of proteins has been the subject of many studies in animals and humans. Plasma proteins can be used as an easy accessible source of specific proteins. In this paper, an innovative method to study the synthetic rate of plasma proteins is described. This methodology, based on the proteomics approach, enables the direct observation of the effects of posttranslational modifications of protein synthesis and/or degradation. The methodology is based on 1D or 2D electrophoresis and subsequent electrospray ionization liquid chromatography mass spectrometry (ESI-LC-MS). Protein synthesis is measured in isotopically labeled peptides of the identified proteins. This innovative method can be used to assess amino acid adequacy and safety by studying protein synthesis and posttranslational modification of plasma proteins in more detail.

References

May 1, 1990·Analytical Chemistry·R D SmithH R Udseth
Feb 22, 1995·Biochimica Et Biophysica Acta·S EraM Sogami
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Apr 1, 1997·Neurochemical Research·M E Hallak, G Bongiovanni
Jan 27, 1999·Analytical Biochemistry·C PapageorgopoulosM K Hellerstein
Apr 23, 1999·Journal of Neuroscience Research·G BongiovanniM E Hallak
Aug 17, 1999·Annual Review of Nutrition·I Tabas
Feb 24, 2000·Neurochemical Research·S FissoloM E Hallak
Apr 27, 2001·Journal of the American Society for Mass Spectrometry·A SarverB W Gibson
Jun 8, 2001·Annual Review of Biochemistry·M MannA Pandey
Jul 4, 2001·Journal of Mass Spectrometry : JMS·A S PeterssonP Roepstorff
Jul 20, 2001·Annals of the New York Academy of Sciences·A H Henschen-Edman
Sep 5, 2001·Molecular Immunology·J E Volanakis
Oct 23, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A A JacksonF Jahoor
Nov 9, 2001·Electrophoresis·H J Issaq
Dec 18, 2001·Journal of Mass Spectrometry : JMS·J Peng, S P Gygi
Mar 7, 2002·Microbes and Infection·Alexander J Szalai
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wayne F Patton
Jun 29, 2002·The Journal of Nutritional Biochemistry·Leonie K. Heilbronn, Peter M. Clifton
Jul 6, 2002·Science·Yong Tae KwonAlexander Varshavsky

❮ Previous
Next ❯

Citations

Jul 9, 2016·Journal of Mass Spectrometry : JMS·Mario Fernández-FernándezJ Ignacio García Alonso
Jun 6, 2018·American Journal of Physiology. Endocrinology and Metabolism·Antoinette MoranK Sreekumaran Nair
Jul 26, 2005·Proteomics·Lynne ThadikkaranJean-Daniel Tissot
Jun 3, 2004·The Journal of Nutrition·William M Rand
Sep 3, 2009·Molecular & Cellular Proteomics : MCP·Nadia RachdaouiStephen F Previs
Jun 3, 2004·The Journal of Nutrition·Padma Maruvada, Sudhir Srivastava

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.