Plasma proteomic analysis of systemic lupus erythematosus patients using liquid chromatography/tandem mass spectrometry with label-free quantification

PeerJ
Rashmi MaddaShir-Ly Huang

Abstract

Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease with unknown etiology. Human plasma is comprised of over 10 orders of magnitude concentration of proteins and tissue leakages. The changes in the abundance of these proteins have played an important role in various human diseases. Therefore, the research objective of this study is to identify the significantly altered expression levels of plasma proteins from SLE patients compared with healthy controls using proteomic analysis. The plasma proteome profiles of both SLE patients and controls were compared. A total of 19 active SLE patients and 12 healthy controls plasma samples were analyzed using high-resolution electrospray ionization liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS) followed by label-free quantification. A total of 19 proteins showed a significant level of expression in the comparative LC-ESI-MS/MS triplicate analysis; among these, 14 proteins had >1.5- to three-fold up-regulation and five had <0.2- to 0.6-fold down-regulation. Gene ontology and DAVID (Database Annotation Visualization, and Integrated Discovery) functional enrichment analysis revealed that these proteins are involved in several important biological proc...Continue Reading

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Citations

Mar 7, 2020·International Reviews of Immunology·Wencong SongYong Dai
May 19, 2021·Journal of the American Society for Mass Spectrometry·Rashmi MaddaWei-Ming Chen

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Methods Mentioned

BETA
protein assay
biopsy

Software Mentioned

PEAKS Studio
Mascot
SPSS
emPAI
PEAKS
ProteinLynx Global Server
Panther
PEAKS Q
Excel

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