Plasma QconCATs reveal a gender-specific proteomic signature in apheresis platelet plasma supernatants

Journal of Proteomics
Monika DzieciatkowskaKirk C Hansen

Abstract

Clinical translation of proteomic technologies is often hampered by technical limitations, including inter-laboratory inconsistencies of label-free derived relative quantification, time-consuming analytical approaches and the subsequent challenge of performing proteomic analyses on large cohorts of subjects. Here we introduce plasma QconCAT-based targeted proteomics, an approach that allows the simultaneous absolute quantitation down to the picogram level of hundreds of proteins in a single liquid chromatography-selected reaction monitoring mass spectrometry run. We demonstrate the robustness of the approach by analyzing apheresis platelet concentrate supernatants at storage day 1 and the end of the shelf life for this blood-derived therapeutic, day 5. The targeted approach was repeatable and robust revealing potential gender-specific signatures across a set of three male and female donors. This technical note represents a proof-of-principle of the application of QconCAT-based MRM strategies to transfusion-medicine relevant issues, such as storage and gender-dependent proteomic signatures in blood-derived therapeutics. Gender differences in the proteome composition of apheresis platelet supernatants have always been postulated,...Continue Reading

References

Dec 15, 2004·Journal of Proteome Research·Robertson CraigRonald C Beavis
Mar 19, 2011·Journal of Proteome Research·Christian K FreseShabaz Mohammed
Sep 2, 2011·Transfusion·Christopher C SillimanKirk C Hansen
Mar 29, 2014·Circulation Research·Julia M BurkhartRené P Zahedi
Apr 2, 2014·Transfusion Medicine Reviews·Michel PrudentLello Zolla
Aug 26, 2014·Molecular & Cellular Proteomics : MCP·Cordula KlockenbuschJuergen Kast

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Citations

Mar 20, 2018·The Journal of Trauma and Acute Care Surgery·Anirban BanerjeeKirk Hansen
May 23, 2018·Expert Review of Proteomics·Christina LooßeChristin Lorenz
Dec 28, 2019·Transfusion·Angelo D'AlessandroPhilip C Spinella
Jul 8, 2020·International Journal of Molecular Sciences·Erica GianazzaElena Tremoli
Apr 17, 2021·Frontiers in Endocrinology·Abu Saleh Md MoinAlexandra E Butler
May 1, 2021·International Journal of Molecular Sciences·Narges HadjesfandiariDana V Devine
May 30, 2021·NPJ Systems Biology and Applications·Yassene MohammedChristoph H Borchers
Oct 4, 2017·Journal of Proteome Research·Alexander S BarrettKirk C Hansen
Feb 18, 2017·Analytical Chemistry·Aliaksandra SikirzhytskayaIgor K Lednev
Jul 23, 2016·Journal of Proteome Research·Justina C WoltersHjalmar P Permentier
Sep 21, 2021·The Journal of Trauma and Acute Care Surgery·Alexander P MortonChristopher C Silliman

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