Plasmalemma hyperpolarity and culture of sense and antisenseabp transgenic tobacco protoplasts

Science in China. Series C, Life Sciences
D NiZ Xia

Abstract

The relationship among transfer and expression of auxin binding protein gene (abp), auxin (NAA)-induced plasmalemma hyperpolarity and sensibility to auxin during protoplast culture was studied by measuring transmembrane potential difference (Em) and culturing the protoplasts of sense and antisenseabp transgenic tobacco. The concentration of NAA inducing the highest degree of hyperpolarity of senseabp transgenic tobacco protoplasts was lower than the control, and in protoplast culture, their sensibility to auxin increased. The concentration of antisenseabp transgenic tobacco protoplasts was higher than the control, and in protoplast culture, their sensibility to auxin decreased. These results demonstrated that ABP synthesized in endoplasmic reticulum needed to transport to cell membrane and functioned there.

Citations

Jan 22, 2015·The Journal of Physical Chemistry. B·Shradhey GuptaPratik Sen
Mar 21, 2020·Annual Review of Plant Biology·Zhi-Hong Xu

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