Plasmalemmal V-H(+)-ATPases regulate intracellular pH in human lung microvascular endothelial cells

Biochemical and Biophysical Research Communications
Jose D RojasR Martinez-Zaguilan

Abstract

The lung endothelium layer is exposed to continuous CO(2) transit which exposes the endothelium to a substantial acid load that could be detrimental to cell function. The Na(+)/H(+) exchanger and HCO(3)(-)-dependent H(+)-transporting mechanisms regulate intracellular pH (pH(cyt)) in most cells. Cells that cope with high acid loads might require additional primary energy-dependent mechanisms. V-H(+)-ATPases localized at the plasma membranes (pmV-ATPases) have emerged as a novel pH regulatory system. We hypothesized that human lung microvascular endothelial (HLMVE) cells use pmV-ATPases, in addition to Na(+)/H(+) exchanger and HCO(3)(-)-based H(+)-transporting mechanisms, to maintain pH(cyt) homeostasis. Immunocytochemical studies revealed V-H(+)-ATPase at the plasma membrane, in addition to the predicted distribution in vacuolar compartments. Acid-loaded HLMVE cells exhibited proton fluxes in the absence of Na(+) and HCO(3)(-) that were similar to those observed in the presence of either Na(+), or Na(+) and HCO(3)(-). The Na(+)- and HCO(3)(-)-independent pH(cyt) recovery was inhibited by bafilomycin A(1), a V-H(+)-ATPase inhibitor. These studies show a Na(+)- and HCO(3)(-)-independent pH(cyt) regulatory mechanism in HLMVE cells ...Continue Reading

References

Feb 1, 1979·Cardiovascular Research·F L Belloni
Oct 23, 1992·Science·R C ZiegelsteinM C Capogrossi
Nov 1, 1991·The American Journal of the Medical Sciences·W L Henrich
Oct 1, 1990·The American Journal of Physiology·A Bidani, S E Brown
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J GilliesR Perona
Oct 11, 1990·The American Journal of Physiology·N EscobalesT A Brock
Aug 1, 1990·Cell Biology International Reports·N M Mozingo, D E Chandler
May 1, 1989·Analytical Biochemistry·D W JungG P Brierley
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E J BowmanK Altendorf
Aug 1, 1986·Journal of Applied Physiology·M ShiraiI Ninomiya
Nov 1, 1968·The American Journal of Physiology·P H Viles, J T Shepherd
Jul 1, 1981·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·R M EffrosP Silverman
Jan 1, 1981·Histochemistry·N SugaiT Oosaki
Oct 1, 1982·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·U S RyanJ W Ryan
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D W NielsonJ A Clements
Apr 1, 1981·Physiological Reviews·A Roos, W F Boron
Dec 1, 1994·The American Journal of Physiology·M Cutaia, N Parks
Feb 3, 1995·The Journal of Biological Chemistry·T NordströmS Grinstein
Jun 1, 1994·Journal of Neurophysiology·S Sánchez-ArmassR J Gillies
Nov 1, 1994·Endocrinology·R Martínez-ZaguilánP B Hoyer
Oct 1, 1993·The American Journal of Physiology·R Martinez-ZaguilanR J Gillies
Sep 1, 1993·The Journal of General Physiology·G GambassiM C Capogrossi
Mar 1, 1996·Clinical & Experimental Metastasis·R Martínez-ZaguilánM J Hendrix
Jul 1, 1996·Proceedings of the Society for Experimental Biology and Medicine·P HsuC W Leffler
Mar 11, 2000·American Journal of Respiratory and Critical Care Medicine·J F HuntB Gaston
Jun 29, 2000·Annual Review of Biochemistry·D W Christianson, J D Cox
Nov 7, 2001·The Journal of Biological Chemistry·B C TrippJ G Ferry
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·L K PutneyD L Barber
Feb 12, 2002·Nature Reviews. Molecular Cell Biology·Tsuyoshi Nishi, Michael Forgac
Jun 26, 2002·Annals of the New York Academy of Sciences·Mark J Post
Nov 21, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Deborah Sterling, Joseph R Casey
Aug 14, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Marisol Ruiz-MeanaJordi Soler-Soler
Feb 6, 2004·American Journal of Physiology. Cell Physiology·Souad R SennouneRaul Martínez-Zaguilán

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Citations

Jun 21, 2008·Histochemistry and Cell Biology·W KummerU Pfeil
Sep 6, 2007·American Journal of Physiology. Endocrinology and Metabolism·Sabine ErdmannKatharina Spanel-Borowski
Nov 21, 2013·Angiogenesis·Sebastian RathStefan Zahler
Aug 12, 2016·American Journal of Physiology. Cell Physiology·Julianna Maria SantosSouad R Sennoune
Jun 24, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Reece P StevensJi Young Lee

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