Plasmapheresis modulates Th1/Th2 imbalance in patients with systemic lupus erythematosus according to measurement of intracytoplasmic cytokines

Autoimmunity
P SoltészE Kiss

Abstract

To examine the possible effect of plasmapheresis on the ratio of Th1/Th2 type cytokine-secreting cells we recruited eight patients with active systemic lupus erythematosus into the present study. They all failed to respond to conventional therapy. A sensitive multiparametric flow cytometric analysis was used for the detection of intracellular IL-4, IL-10 and IFNgamma. Stimulated peripheral blood cells were analysed by this procedure. Plasmapheresis was performed every second day for three occasions, using a continuous flow type blood cell separator, and a total of 100 ml/body weight kg plasma was removed. Patients received 1 mg/kg/day methylprednisolone during this period. As a result of the procedure, the rate of IFNgamma positive Th cells increased, while the rate of IL-4 and IL-10 expressing CD4 positive cells decreased. Together with these observations the concentration of anti-ds-DNA antibodies decreased after plasmapheresis. A decrease in disease activity index (SLE-DAI) indicated the clinical effectiveness of the therapy.

Citations

Mar 16, 2016·Medicina clínica·María M Trujillo-MartínUNKNOWN en nombre del Grupo Elaborador de la GPC-LES
Nov 1, 2013·British Journal of Haematology·Hollie M Reeves, Jeffrey L Winters
Jul 8, 2008·Autoimmunity Reviews·Maria Kavai
Apr 14, 2007·Orvosi hetilap·János JákóPál Soltész
Apr 18, 2013·Transplantation·Mahmoud SadeghiGerhard Opelz
Apr 23, 2003·Current Opinion in Rheumatology·Yolanda Braun-Moscovici, Daniel E Furst
Sep 1, 2007·Expert Review of Clinical Immunology·Michael CentolaPeter Szodoray
Mar 28, 2008·Expert Review of Molecular Diagnostics·Chang-Hee Suh, Hyoun-Ah Kim
Nov 22, 2019·World Journal of Transplantation·Maurizio Salvadori, Aris Tsalouchos
Nov 11, 2019·La Presse médicale·Maria Prendecki, Charles Pusey
Jun 5, 2021·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Melod MehdipourDobri D Kiprov
Aug 24, 2021·Annals of Neurology·Kimitoshi KimuraTakashi Yamamura

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