Plasmid-based Survivin shRNA and GRIM-19 carried by attenuated Salmonella suppresses tumor cell growth.

Asian Journal of Andrology
Yan-Bo LiuDe-Qi Xu

Abstract

Persistent activation of Survivin and its overexpression contribute to the formation, progression and metastasis of several different tumor types. Therefore, Survivin is an ideal target for RNA interference mediated-growth inhibition. Blockade of Survivin using specific short hairpin RNAs (shRNA) can significantly reduce prostate tumor growth. RNA interference does not fully ablate target gene expression, owing to the idiosyncrasies associated with shRNAs and their targets. To enhance the therapeutic efficacy of Survivin-specific shRNA, we employed a combinatorial expression of Survivin-specific shRNA and gene associated with retinoid-interferon-induced mortality-19 (GRIM-19). Then, the GRIM-19 coding sequences and Survivin-specific shRNAs were used to create a dual expression plasmid vector and were carried by an attenuated strain of Salmonella enteric serovar typhimurium (S. typhimurium) to treat prostate cancer in vitro and in vivo. We found that the co-expressed Survivin-specific shRNA and GRIM-19 synergistically and more effectively inhibited prostate tumor proliferation and survival, when compared with treatment with either single agent alone in vitro and in vivo. This study has provided a novel cancer gene therapeutic ap...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A CarswellB Williamson
Dec 2, 1993·International Journal of Cancer. Journal International Du Cancer·P PisaniJ Ferlay
Aug 1, 1997·Nature Medicine·G AmbrosiniD C Altieri
Aug 28, 1998·Molecular Microbiology·S A KhanD J Maskell
Aug 17, 1999·International Journal of Cancer. Journal International Du Cancer·P PisaniJ Ferlay
Dec 2, 1999·Nature Genetics·V E VelculescuK W Kinzler
Dec 2, 1999·Nature Genetics·E PassargeR A Farber
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Sayda M ElbashirThomas Tuschl
Nov 16, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jiadi HuDhananjaya V Kalvakolanu
Sep 26, 2003·Cancer·Sharon H Giordano
Jul 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tobias N DechowJacqueline F Bromberg
Jan 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ming ZhaoRobert M Hoffman
Sep 8, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lifang GaoDe-Qi Xu
Mar 25, 2006·Journal of Cellular Physiology·Fengzhi Li, Xiang Ling
Dec 16, 2006·Journal of Molecular Endocrinology·Niamh CosgraveLeonie S Young
Jun 15, 2007·International Journal of Oral and Maxillofacial Surgery·M A ScheperJ J Sauk
Jan 29, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ling ZhangDe-Qi Xu
Mar 24, 2009·Methods in Molecular Biology·De-Qi XuLijing Zhao
Mar 28, 2009·Methods : a Companion to Methods in Enzymology·Margret Schottelius, Hans-Jürgen Wester
Jan 19, 2010·Chinese Journal of Integrative Medicine·Yan-zhi ChenPing-ping Li

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Citations

May 5, 2016·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Ying ZhouWeihua Xiao
Sep 24, 2016·Cytokine & Growth Factor Reviews·Shreeram C Nallar, Dhan V Kalvakolanu
Sep 19, 2019·Clinical and Experimental Pharmacology & Physiology·Haili LinHong Chen
Oct 1, 2016·Chonnam Medical Journal·Jin Hai Zheng, Jung-Joon Min
Mar 18, 2016·International Journal of Oncology·Kebang HuLijing Zhao
Nov 9, 2018·Nature Reviews. Cancer·Shibin ZhouKe Liu

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